Crystallization (Comment) | Organism |
---|---|
purified enzyme in complex with substrate, hanging drop vapour diffusion method, 20°C, mixing of 0.0015 ml of protein solution containing 12 mg/ml in 10 mM HEPES-NaOH, pH 7.5, and 3 mM NaN3 with 0.0015 ml of reservoir solution containing 10-17% w/v PEG 4000, 0.1 M BESNaOH, pH 6.5, cryoprotection by 17% w/v PEG 4000, 10% v/v PEG 400, 0.1 MBESNaOH, pH 6.5, X-ray diffraction structure determination and analysis at 2.05 A resolution, molecular replacement | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | Thermotoga maritima | - |
galacturonic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | modelling of substrate binding, and active site structure, overview | Thermotoga maritima | ? | - |
? | |
polygalacturonic acid + H2O | - |
Thermotoga maritima | galacturonic acid + ? | - |
? | |
tetragalacturonic acid + H2O | modelling of substrate binidng, structure, overview | Thermotoga maritima | galacturonic acid + trigalacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | quaternary structure, overview | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
exoPG | - |
Thermotoga maritima |
exopolygalacturonase | - |
Thermotoga maritima |
More | the enzyme belongs to the glycosyl hydrolase family 28, GH28 | Thermotoga maritima |