Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Caldicellulosiruptor sp. F32 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 10 mM, 77% residual activity | Caldicellulosiruptor sp. F32 | |
EDTA | 10 mM, 63% residual activity | Caldicellulosiruptor sp. F32 | |
Mn2+ | 1 mM, 56% of initial activity | Caldicellulosiruptor sp. F32 | |
SDS | 10 mM, 70% residual activity | Caldicellulosiruptor sp. F32 | |
Urea | 10 mM, 85% residual activity | Caldicellulosiruptor sp. F32 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | Km value 1.38 mg/ml, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
additional information | - |
Laminarin | Km value 2.63 mg/ml, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 119% of initial activity | Caldicellulosiruptor sp. F32 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor sp. F32 | A0A0S2UQ60 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Caldicellulosiruptor sp. F32 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
172 | - |
substrate laminarin, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |
2961 | - |
substrate barley beta-glucan, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Caldicellulosiruptor sp. F32 | ? | - |
? | |
beta-1,3-1,4-D-glucotriose + H2O | - |
Caldicellulosiruptor sp. F32 | D-glucose + cellobiose | - |
? | |
laminarin + H2O | - |
Caldicellulosiruptor sp. F32 | ? | - |
? | |
additional information | enzyme hydrolyzes both beta-1,3-glucan (laminarin) and beta-1,3-1,4-glucan linkages, catalyzes endo-hydrolysis of the beta-1,4 glycosidic linkage adjacent to a 3-O-substituted glucosyl residues in the mixed linked beta-glucans, as well as the beta-1,3 linkage. No substrates: carboxymethylcellulose, avicel | Caldicellulosiruptor sp. F32 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lam16A | - |
Caldicellulosiruptor sp. F32 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Caldicellulosiruptor sp. F32 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
half-life 45 h | Caldicellulosiruptor sp. F32 |
85 | - |
30 min, 20% residual activity | Caldicellulosiruptor sp. F32 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Caldicellulosiruptor sp. F32 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Laminarin | kcat/Km value 102 ml/mg/s, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 | |
additional information | - |
Barley beta-glucan | kcat/Km value 2116 ml/mg/s, pH 6.5, 75°C | Caldicellulosiruptor sp. F32 |