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Literature summary for 3.2.1.58 extracted from

  • Zhou, J.; Li, Z.; Wu, J.; Li, L.; Li, D.; Ye, X.; Luo, X.; Huang, Y.; Cui, Z.; Cao, H.
    Functional Analysis of a novel beta-(1,3)-glucanase from Corallococcus sp. strain EGB containing a fascin-like module (2017), Appl. Environ. Microbiol., 83, e01016-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.58

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Origami B(DE3) cells Corallococcus sp. EGB

Protein Variants

Protein Variants Comment Organism
E304A inactive Corallococcus sp. EGB
E309A inactive Corallococcus sp. EGB

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Corallococcus sp. EGB most favorable substrate D-glucose + ?
-
 ?
pachyman + H2O Corallococcus sp. EGB
-
 D-glucose + ?
-
 ?
pustulan + H2O Corallococcus sp. EGB
-
 D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Corallococcus sp. EGB
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Corallococcus sp. EGB

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O most favorable substrate Corallococcus sp. EGB D-glucose + ?
-
 ?
pachyman + H2O
-
 Corallococcus sp. EGB D-glucose + ?
-
 ?
pustulan + H2O
-
 Corallococcus sp. EGB D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47040, calculated from amino acid sequence Corallococcus sp. EGB

Synonyms

Synonyms Comment Organism
beta-(1,3)(1,3)-glucanase
-
 Corallococcus sp. EGB
beta-(1,3)-glucanase
-
 Corallococcus sp. EGB
lamC
-
 Corallococcus sp. EGB
lamC the enzyme shows exo-beta-(1,3)/(1,6)-glucanase and displays and endo-beta-(1,4)-glucanase/xylanase activities Corallococcus sp. EGB
laminarinase
-
 Corallococcus sp. EGB

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Corallococcus sp. EGB

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 the enzyme shows about 70% activity at 30°C, about 75% activity at 35°C, about 85% activity at 40°C, about 90% activity at 45°C, 100% activity at 50°C, about 88% activity at 55°C, about 65% activity at 60°C, about 40% activity at 65°C, and about 25% activity at 70°C Corallococcus sp. EGB

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme retains more than 80% of its original activity after 30 min of incubation at 50°C Corallococcus sp. EGB

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Corallococcus sp. EGB

pH Range

pH Minimum pH Maximum Comment Organism
6 8 the enzyme shows more than 75% of activity between pH 6.0 and 8.0 Corallococcus sp. EGB

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 the enzyme retains more than 60% of its original activity after 30 min of incubation at pH 6.0-8.0 Corallococcus sp. EGB

pI Value

Organism Comment pI Value Maximum pI Value
Corallococcus sp. EGB isoelectric focusing
-
 5.3