Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant E282S alone or in complex with alpha-D-glucopyranosyl fluoride and 4-nitrophenyl-beta-D-glucopyranoside, or with 4-nitrophenyl-gentiobiose, X-ray diffraction structure determination and analysis at 1.59-1.96 A resolution | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
E292G | site-directed mutagenesis | Candida albicans |
E292S | site-directed mutagenesis, the mutant shows altered transglycosidase activity as the wild-type enzyme and displays modest glycosynthase activity with alpha-D-glucopyranosyl fluoride as the donor and 4-nitrophenyl-beta-D-glucopyranoside as the acceptor, but surprisingly shows a marked preference for synthesizing beta-1,6-linked over beta-1,3- and beta-1,4-linked disaccharide products. Mechanism and expected regiospecificity for the glycosynthase reaction, overview | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | P29717 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows glycohydrolase and transglycosidase activities, substrate binding structures determined from mutant E292S complex crystal structures, detailed overview | Candida albicans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exg | - |
Candida albicans |
exo-1,3-beta-glucanase | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Candida albicans |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in cell wall beta-D-glucan metabolism and morphogenesis through its hydrolase and transglycosidase activities | Candida albicans |