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Literature summary for 3.2.1.58 extracted from

  • Kulminskaya, A.A.; Thomsen, K.K.; Shabalin, K.A.; Sidorenko, I.A.; Eneyskaya, E.V.; Savel'ev, A.N.; Neustroev, K.N.
    Isolation, enzymatic properties, and mode of action of an exo-1,3-beta-glucanase from Trichoderma viride (2001), Eur. J. Biochem., 268, 6123-6131.
    View publication on PubMed
Search for more literature for 3.2.1.58

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 81% of activity Trichoderma viride
Hg2+ 1 mM, 86% of activity Trichoderma viride
Zn2+ 1 mM, 83% of activity Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
2
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
2.1
-
 beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
5
-
 beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride
16.5
-
 beta-D-glucose-1,3-beta-D-glucoside pH 4.5, 37°C Trichoderma viride

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma viride
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 136% of activity Trichoderma viride

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Trichoderma viride

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma viride

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63
-
 pH 4.5, 37°C Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
beta-D-glucose-beta-D-glucose-1,3-beta-D-glucose-1,3-beta-D-glucose-beta-D-glucose-1,3-beta-D-glucoside + H2O
-
 Trichoderma viride ?
-
 ?
curdlan + H2O
-
 Trichoderma viride ?
-
 ?
laminarin + H2O
-
 Trichoderma viride ?
-
 ?
additional information hydrolysis with inversion of the anomeric configuration, substrate subsite structure and model of enzyme binding site Trichoderma viride ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Trichoderma viride
More N-terminal sequence Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 pH 3.5-7.5, stable for 24 h Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Trichoderma viride