Any feedback?
Literature summary for 3.2.1.55 extracted from
- Identification and characterization of a novel galactofuranose-specific beta-D-galactofuranosidase from Streptomyces species (2015), PLoS ONE, 10, e0137230 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| orf0232, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces viridochromogenes |
| orf2125, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces chartreusis |
| orf2812, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces sp. JHA19 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces chartreusis | A0A0K2SQX3 | isolated from soil in Kagawa University, Japan | - |
| Streptomyces chartreusis JHA19 | A0A0K2SQX3 | isolated from soil in Kagawa University, Japan | - |
| Streptomyces sp. JHA19 | A0A0K2SQZ9 | isolated from soil in Kagawa University, Japan | - |
| Streptomyces sp. JHA19 NRRL F-5140 | A0A0K2SQZ9 | isolated from soil in Kagawa University, Japan | - |
| Streptomyces viridochromogenes | A0A0K2SQX4 | isolated from soil in Kagawa University, Japan | - |
| Streptomyces viridochromogenes JHA19 | A0A0K2SQX4 | isolated from soil in Kagawa University, Japan | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces chartreusis |
| recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces sp. JHA19 |
| recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces chartreusis | 4-nitrophenol + L-arabinofuranose | - |
? |  |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces viridochromogenes | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. JHA19 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces chartreusis JHA19 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. JHA19 NRRL F-5140 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces viridochromogenes JHA19 | 4-nitrophenol + L-arabinofuranose | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces chartreusis | ? | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces viridochromogenes | ? | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces sp. JHA19 | ? | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces chartreusis JHA19 | ? | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces sp. JHA19 NRRL F-5140 | ? | - |
? | |
| additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces viridochromogenes JHA19 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Araf-ase | - |
Streptomyces chartreusis |
| Araf-ase | - |
Streptomyces viridochromogenes |
| Araf-ase | - |
Streptomyces sp. JHA19 |
| beta-D-galactofuranosidase 1 | UniProt | Streptomyces viridochromogenes |
| beta-D-galactofuranosidase 3 | UniProt | Streptomyces chartreusis |
| beta-D-galactofuranosidase 4 | UniProt | Streptomyces sp. JHA19 |
| Galf-ase | - |
Streptomyces chartreusis |
| Galf-ase | - |
Streptomyces viridochromogenes |
| Galf-ase | - |
Streptomyces sp. JHA19 |
| More | see also EC 3.2.1.146 | Streptomyces chartreusis |
| More | see also EC 3.2.1.146 | Streptomyces viridochromogenes |
| More | see also EC 3.2.1.146 | Streptomyces sp. JHA19 |
| ORF0232 | locus name | Streptomyces viridochromogenes |
| ORF2125 | locus name | Streptomyces chartreusis |
| ORF2812 | locus name | Streptomyces sp. JHA19 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces chartreusis |
| 37 | - |
assay at | Streptomyces viridochromogenes |
| 37 | - |
assay at | Streptomyces sp. JHA19 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Streptomyces chartreusis |
| 5.5 | - |
assay at | Streptomyces viridochromogenes |
| 5.5 | - |
assay at | Streptomyces sp. JHA19 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | four genes in Streptomyces sp. JHA19 encode beta-D-galactofuranosidase (Galf-ases). One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces sp. JHA19 |
| evolution | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces chartreusis |
| evolution | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces viridochromogenes |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
