Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Pleurotus ostreatus |
Protein Variants | Comment | Organism |
---|---|---|
S160G | catalytic properties are slightly changed. The mutation negatively affects the stability of the enzyme at various pHs and temperatures. Circular dichroism analyses show a minimum at 210 nm for wild-type, which is shifted for mutant S160G, suggesting the presence of an unfolded structure, with a role of the glycosylation at the S160 residue for secondary structure stability | Pleurotus ostreatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.64 | - |
4-nitrophenyl alpha-L-arabinofuranoside | wild-type, pH 5.0, 40°C | Pleurotus ostreatus | |
0.89 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant S160G, pH 5.0, 40°C | Pleurotus ostreatus | |
4.1 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.0, 40°C | Pleurotus ostreatus | |
5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant S160G, pH 5.0, 40°C | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | G0TES6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinohexaose + H2O | - |
Pleurotus ostreatus | 1,5-alpha-L-arabinopentaose + L-arabinose | - |
? | |
1,5-alpha-L-arabinotriose + H2O | - |
Pleurotus ostreatus | 1,5-alpha-L-arabinobiose + L-arabinose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Pleurotus ostreatus | 4-nitrophenol + L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pleurotus ostreatus | 4-nitrophenol + D-glucose | - |
? | |
carboxymethyl linear arabinan + H2O | - |
Pleurotus ostreatus | L-arabinose + ? | - |
? | |
additional information | no substrates: 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-galactopyranoside. In presence of AZO-wheat arabinoxylan, the enzyme dispalys an endo-1,4-beta-xylanase activity | Pleurotus ostreatus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pleurotus ostreatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life for wild-type 16 days, for mutant S160G 7 days | Pleurotus ostreatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant S160G, pH 5.0, 40°C | Pleurotus ostreatus | |
0.38 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.0, 40°C | Pleurotus ostreatus | |
43 | - |
4-nitrophenyl alpha-L-arabinofuranoside | mutant S160G, pH 5.0, 40°C | Pleurotus ostreatus | |
50 | - |
4-nitrophenyl alpha-L-arabinofuranoside | wild-type, pH 5.0, 40°C | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pleurotus ostreatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
citrate-phosphate buffer, half-life for wild-type 38 days, for mutant S160G 16 days | Pleurotus ostreatus |