Any feedback?
Literature summary for 3.2.1.55 extracted from
- Identification of a GH62 alpha-L-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum (2011), Appl. Microbiol. Biotechnol., 90, 137-146.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Penicillium chrysogenum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32827 | - |
x * 32827, calculated from amino acid sequence | Penicillium chrysogenum |
| 35000 | - |
gel filtration | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | B5MGR2 | - |
- |
| Penicillium chrysogenum 31B | B5MGR2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Mono Q column chromatography, and Mono S column chromatography. Ni-Sepharose 6 column chromatography | Penicillium chrysogenum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |  |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Penicillium chrysogenum 31B | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| insoluble wheat arabinoxylan + H2O | about 28% activity compared to soluble wheat arabinoxylan, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones | Penicillium chrysogenum | L-arabinose | L-arabinose is detected as the only product | ? | |
| insoluble wheat arabinoxylan + H2O | about 28% activity compared to soluble wheat arabinoxylan, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones | Penicillium chrysogenum 31B | L-arabinose | L-arabinose is detected as the only product | ? | |
| additional information | 4-nitrophenyl beta-D-xylopyranoside, alpha-1,5-arabinofuranobiose, sugar beet L-arabinan, and birch wood xylan are resistant to the action of recombinant enzyme even when the enzyme is present in excess | Penicillium chrysogenum | ? | - |
? | |
| additional information | no activity with branched sugar beet arabinan | Penicillium chrysogenum | ? | - |
? | |
| additional information | 4-nitrophenyl beta-D-xylopyranoside, alpha-1,5-arabinofuranobiose, sugar beet L-arabinan, and birch wood xylan are resistant to the action of recombinant enzyme even when the enzyme is present in excess | Penicillium chrysogenum 31B | ? | - |
? | |
| additional information | no activity with branched sugar beet arabinan | Penicillium chrysogenum 31B | ? | - |
? | |
| soluble wheat arabinoxylan + H2O | best substrate, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones | Penicillium chrysogenum | L-arabinose | L-arabinose is detected as the only product | ? | |
| soluble wheat arabinoxylan + H2O | best substrate, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones | Penicillium chrysogenum 31B | L-arabinose | L-arabinose is detected as the only product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32827, calculated from amino acid sequence | Penicillium chrysogenum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AFase | - |
Penicillium chrysogenum |
| AFS1 | - |
Penicillium chrysogenum |
| alpha-L-arabinofuranosidase | - |
Penicillium chrysogenum |
| arabinoxylan arabinofuranohydrolase | - |
Penicillium chrysogenum |
| AXS5 | - |
Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Penicillium chrysogenum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | after 1 h pre-incubation of the enzyme in 20 mM Na-acetate buffer (pH 5.0), the enzyme is stable up to 40°C and loses all activity by treatment at 60°C | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium chrysogenum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Penicillium chrysogenum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium chrysogenum | calculated from amino acid sequence | - |
4.7 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Penicillium chrysogenum | expression of the axs5 gene is strongly induced by adding D-xylose and arabinoxylan to the culture medium | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | arabinoxylan arabinofuranohydrolase AXS5 is involved in xylan degradation | Penicillium chrysogenum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
