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Literature summary for 3.2.1.55 extracted from

  • Shin, H.Y.; Park, S.Y.; Sung, J.H.; Kim, D.H.
    Purification and characterization of alpha-L-arabinopyranosidase and alpha-L-arabinofuranosidase from Bifidobacterium breve K-110, a human intestinal anaerobic bacterium metabolizing ginsenoside Rb2 and Rc (2003), Appl. Environ. Microbiol., 69, 7116-7123.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.55

Activating Compound

Activating Compound Comment Organism Structure
Ba2+ slight activation at 1 mM Bifidobacterium breve
Co2+ slight activation at 1 mM Bifidobacterium breve

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition at 1 mM Bifidobacterium breve
Li+ slight inhibition at 1 mM Bifidobacterium breve
additional information no inhibition by PMSF, tosyl-L-lysine chloromethyl ketone, iodoacetic acid, paraoxon, DTT, 2-mercaptoethanol, 2,3-butanediol, NEM, and carbodiimide Bifidobacterium breve
Ni2+ slight inhibition at 1 mM Bifidobacterium breve
p-chloromercuriphenylsulfonic acid strong inhibition at 1 mM Bifidobacterium breve

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.084
-
 ginsenoside Rc pH 7.0, 37°C Bifidobacterium breve
0.22
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 7.0, 37°C Bifidobacterium breve
1.01
-
 4-nitrophenyl-alpha-L-arabinopyranoside pH 7.0, 37°C Bifidobacterium breve

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Bifidobacterium breve

Organism

Organism UniProt Comment Textmining
Bifidobacterium breve
-
 isolated from human intestinal microflora
-
Bifidobacterium breve K-110
-
 isolated from human intestinal microflora
-

Purification (Commentary)

Purification (Comment) Organism
59fold to homogeneity by ammonium sulfate precipitation and 4 chromatographic steps Bifidobacterium breve

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.46
-
 purified enzyme Bifidobacterium breve

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium breve 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Bifidobacterium breve K-110 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O low activity Bifidobacterium breve 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O low activity Bifidobacterium breve K-110 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
ginsenoside Rc + H2O
-
 Bifidobacterium breve ?
-
 ?
ginsenoside Rc + H2O
-
 Bifidobacterium breve K-110 ?
-
 ?
additional information substrate specificity, no activity with ginsenoside Rb2, Ra1, and Ra2, no activity with 4-nitrophenyl-beta-galactopyranoside or 4-nitrophenyl-beta-D-fucopyranoside, no activity with arabinogalactan, overview Bifidobacterium breve ?
-
 ?
additional information substrate specificity, no activity with ginsenoside Rb2, Ra1, and Ra2, no activity with 4-nitrophenyl-beta-galactopyranoside or 4-nitrophenyl-beta-D-fucopyranoside, no activity with arabinogalactan, overview Bifidobacterium breve K-110 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Bifidobacterium breve

Synonyms

Synonyms Comment Organism
alpha-L-arabinofuranosidase
-
 Bifidobacterium breve

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bifidobacterium breve

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Bifidobacterium breve