Application | Comment | Organism |
---|---|---|
paper production | - |
Aspergillus niger |
paper production | extraction of lignin from wood chips, essential step in the preparation of pulp fibres | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
57500 | - |
1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
108000 | - |
gel filtration, Sepharose Cl 6B | Geobacillus stearothermophilus |
110000 | - |
gel filtration | Geobacillus stearothermophilus |
114800 | - |
gel filtration, Superose 12 | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
softwood Kraft pulp + H2O | Geobacillus stearothermophilus | - |
lignin + ? | - |
? | |
softwood Kraft pulp + H2O | Geobacillus stearothermophilus L1 | - |
lignin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Geobacillus stearothermophilus | - |
L1 | - |
Geobacillus stearothermophilus L1 | - |
L1 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101 | - |
- |
Geobacillus stearothermophilus |
126 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | low activity on the substrate | Geobacillus stearothermophilus | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | low activity on the substrate | Geobacillus stearothermophilus L1 | L-arabinose + ? | - |
? | |
L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | low activity on the substrate | Geobacillus stearothermophilus | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | low activity on the substrate | Geobacillus stearothermophilus L1 | L-arabinose + ? | - |
ir | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus L1 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus stearothermophilus L1 | L-arabinose + phenol | - |
ir | |
softwood Kraft pulp + H2O | - |
Geobacillus stearothermophilus | lignin + ? | - |
? | |
softwood Kraft pulp + H2O | - |
Geobacillus stearothermophilus L1 | lignin + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 52500 + 1 * 57500, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
at pH 7 and pH 8 | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | at pH 7 enzyme is completely stable at 60°C for at least 80 min, retains 50% of its maximum activity after 75 min at 70°C and loses all activity after 15 min at 80°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Geobacillus stearothermophilus |