KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces purpurascens | |
0.26 | - |
beet arabinan | - |
Aspergillus niger | |
1.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Ruminococcus albus | |
2 | - |
beet arabinan | - |
Clostridium acetobutylicum | |
3.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces sp. | |
4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Ruminococcus albus | |
4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Clostridium acetobutylicum | |
4.9 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
extracellular | - |
Ruminococcus albus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
- |
Aspergillus niger |
62000 | - |
8 * 62000 | Streptomyces purpurascens |
65000 | - |
- |
Bacillus subtilis |
75000 | - |
4 * 75000, SDS-PAGE | Ruminococcus albus |
90000 | - |
SDS-PAGE | Clostridium acetobutylicum |
92000 | - |
- |
Streptomyces sp. |
94000 | - |
gel filtration | Clostridium acetobutylicum |
310000 | - |
- |
Ruminococcus albus |
495000 | - |
- |
Streptomyces purpurascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Ruminococcus albus | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Streptomyces sp. | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Streptomyces purpurascens | - |
L-arabinose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Clostridium acetobutylicum | - |
ATCC 824 | - |
Ruminococcus albus | - |
- |
- |
Streptomyces purpurascens | - |
- |
- |
Streptomyces sp. | - |
no. 17-1 | - |
Streptomyces sp. 17-1 | - |
no. 17-1 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Ruminococcus albus |
- |
Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.8 | - |
p-nitrophenyl-alpha-D-glucoside as substrate | Clostridium acetobutylicum |
19.2 | - |
- |
Clostridium acetobutylicum |
19.4 | - |
p-nitrophenyl-alpha-L-arabinofuranoside as substrate | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-alpha-L-arabinobiose + H2O | - |
Streptomyces purpurascens | L-arabinose | - |
? | |
1,5-alpha-L-arabinotriose + 2 H2O | - |
Aspergillus niger | 3 L-arabinose | - |
? | |
1,5-alpha-L-arabinotriose + 2 H2O | - |
Streptomyces purpurascens | 3 L-arabinose | - |
? | |
4-nitrophenyl alpha-D-glucoside + H2O | 9% of the rate exhibited on p-nitrophenyl-alpha-L-arabinofuranoside | Clostridium acetobutylicum | 4-nitrophenol + D-glucose | - |
? | |
base-soluble carbohydrates + H2O | BSC of alfalfa cell walls | Ruminococcus albus | ? | - |
? | |
beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Ruminococcus albus | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Streptomyces purpurascens | L-arabinose + ? | - |
? | |
L-arabinan + H2O | - |
Bacillus subtilis | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Clostridium acetobutylicum | (1,5-alpha-L-arabinose)25 + ? | - |
ir | |
L-arabinan + H2O | - |
Streptomyces purpurascens | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
? | |
L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
ir | |
additional information | - |
Bacillus subtilis | ? | - |
? | |
additional information | - |
Aspergillus niger | ? | - |
? | |
additional information | - |
Streptomyces sp. | ? | - |
? | |
additional information | - |
Streptomyces purpurascens | ? | - |
? | |
additional information | no activity against p-nitrophenylglycosides, except p-nitrophenyl-alpha-D-glucoside, no activity on carboxymethylcellulose, arabinogalactan, arabinoxylan or oat spelt xylan, larchwood xylan, CMC and rye arabinoxylan are not degraded | Clostridium acetobutylicum | ? | - |
? | |
additional information | - |
Streptomyces sp. 17-1 | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Ruminococcus albus | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + phenol | - |
ir | |
polygalacturonase-soluble carbohydrates + H2O | PGSC of alfalfa cell walls | Ruminococcus albus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Bacillus subtilis |
monomer | - |
Aspergillus niger |
monomer | - |
Streptomyces sp. |
monomer | 1 * 90000, SDS-PAGE | Clostridium acetobutylicum |
octamer | 8 * 62000 | Streptomyces purpurascens |
tetramer | 4 * 75000, SDS-PAGE | Ruminococcus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.8 | 4 | - |
Aspergillus niger |
5 | 5.5 | - |
Clostridium acetobutylicum |
6 | - |
- |
Streptomyces sp. |
6.5 | - |
- |
Bacillus subtilis |
6.5 | - |
- |
Streptomyces purpurascens |
6.9 | - |
- |
Ruminococcus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | poor stability at low pH | Clostridium acetobutylicum |
4 | 9 | - |
Streptomyces sp. |
5.5 | 8 | - |
Clostridium acetobutylicum |