Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | recombinant enzyme | Paenibacillus sp. |
Cloned (Comment) | Organism |
---|---|
expression by using the pUC 18 vector in Escherichia coli JM 109 | Paenibacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide | 5 mM, complete inhibition | Paenibacillus sp. | |
AgNO3 | 10 mM, complete inhibition | Paenibacillus sp. | |
BaCl2 | 10 mM, 12% inhibition | Paenibacillus sp. | |
diethyldicarbonate | 1 mM, complete inhibition, recombinant enzyme | Paenibacillus sp. | |
HgCl2 | 10 mM, complete inhibition | Paenibacillus sp. | |
iodoacetamide | 5 mM, 29% inhibition | Paenibacillus sp. | |
MgCl2 | 10 mM, 20% inhibition | Paenibacillus sp. | |
N-bromosuccinimide | 0.1 mM, 95% inhibition, recombinant enzyme | Paenibacillus sp. | |
Phenylglyoxal | 5 mM, 38% inhibition | Paenibacillus sp. | |
ZnCl2 | 10 mM, 63% inhibition | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
beta-cyclodextrin | - |
Paenibacillus sp. | |
2.9 | - |
gamma-cyclodextrin | - |
Paenibacillus sp. | |
5 | - |
alpha-cyclodextrin | - |
Paenibacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | recombinant enzyme is produced in Escherichia coli primarily in | Paenibacillus sp. | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
- |
- |
Paenibacillus sp. A11 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | 57% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | + less amounts of maltotriose and maltotetraose | ? | |
beta-cyclodextrin + H2O | - |
Paenibacillus sp. | D-glucose + maltose | + less amounts of maltotriose and maltotetraose | ? | |
gamma-cyclodextrin + H2O | 72% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose | + less amounts of maltotriose and maltotetraose | ? | |
maltoheptaose + H2O | 43% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose + ? | - |
? | |
maltoheptaose + H2O | 43% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | D-glucose + maltose + ? | - |
? | |
maltohexaose + H2O | 35% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose + ? | - |
? | |
maltohexaose + H2O | 35% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | D-glucose + maltose + ? | - |
? | |
maltopentaose + H2O | 22% of the activity with beta-cyclodextrin | Paenibacillus sp. | D-glucose + maltose + ? | - |
? | |
maltopentaose + H2O | 22% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | D-glucose + maltose + ? | - |
? | |
maltotetraose + H2O | 14% of the activity with beta-cyclodextrin | Paenibacillus sp. | 2 D-glucose + maltose | - |
? | |
maltotetraose + H2O | 14% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | 2 D-glucose + maltose | - |
? | |
maltotriose + H2O | 11% of the activity with beta-cyclodextrin | Paenibacillus sp. | maltose + D-glucose | - |
? | |
maltotriose + H2O | 11% of the activity with beta-cyclodextrin | Paenibacillus sp. A11 | maltose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, SDS-PAGE | Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Paenibacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
alpha-cyclodextrin | - |
Paenibacillus sp. | |
26.7 | - |
gamma-cyclodextrin | - |
Paenibacillus sp. | |
36.7 | - |
beta-cyclodextrin | - |
Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Paenibacillus sp. |