Application | Comment | Organism |
---|---|---|
medicine | the differentiation of HT-29 colon cancer cells can be used as a model to study the alteration of the enzyme alpha-L-fucosidase during the progression of the tumoural processs | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-methylumbelliferyl alpha-L-fucopyranoside | pH 5.0, 37°C, undifferentiated HT-29 cells | Homo sapiens | |
0.07 | - |
4-methylumbelliferyl alpha-L-fucopyranoside | pH 5.0, 37°C, differentiated HT-29 cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HT-29 cell | cell colorectal cancer cells | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
variation of activity during differentiation | Homo sapiens |
0.003 | - |
non-differentiated HT-29 cells | Homo sapiens |
0.009 | - |
differentiated HT-29 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of elution profile of enzyme from undifferentiated and differentiated HT-29 cells through gel filtration and SDS-PAGE, presence of a 50000 Da protein in both cultures | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of heat stability of enzyme from undifferentiated and differentiated HT-29 cells | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
comparison of pH stability of enzyme from undifferentiated and differentiated HT-29 cells, enzymes from undifferentiated and differentiated HT-29 cells are very stable at neutral and basic pH values | Homo sapiens |