Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
extracellular | - |
Penicillium multicolor | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Penicillium multicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine | Penicillium multicolor | same yield of transglycosylation and hydrolysis products | alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose | - |
? | |
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine | Penicillium multicolor | slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity | alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose | - |
? | |
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine | Penicillium multicolor | same yield of transglycosylation and hydrolysis products | alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose | - |
? | |
Fuc-alpha-(1-3)-GlcNAc + H2O | Aspergillus niger | slow hydrolysis rate | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
Fuc-alpha-(1-3)-GlcNAc + H2O | Penicillium multicolor | slow hydrolysis rate | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl formamide | - |
Aspergillus niger |
dimethyl formamide | more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v | Penicillium multicolor |
dimethyl sulfoxide | - |
Aspergillus niger |
dimethyl sulfoxide | more stable than the Aspergillus niger enzyme, no loss of activity up to 20%, v/v | Penicillium multicolor |
dioxane | - |
Aspergillus niger |
dioxane | more stable than the Aspergillus niger enzyme, no loss of activity up to 10%, v/v | Penicillium multicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Penicillium multicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Penicillium multicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | lactase-P | Penicillium multicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
- |
Penicillium multicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O | transglycosylation activity | Aspergillus niger | 4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc | no other isomer | ? | |
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O | transglycosylation activity | Penicillium multicolor | 4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc | no other isomer | ? | |
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine | same yield of transglycosylation and hydrolysis products | Penicillium multicolor | alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose | - |
? | |
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine | slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity | Penicillium multicolor | alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose | - |
? | |
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine | same yield of transglycosylation and hydrolysis products | Penicillium multicolor | alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose | - |
? | |
Fuc-alpha-(1-3)-GlcNAc + H2O | slow hydrolysis rate | Aspergillus niger | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
Fuc-alpha-(1-3)-GlcNAc + H2O | slow hydrolysis rate | Penicillium multicolor | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable up to | Aspergillus niger |
50 | - |
stable up to | Penicillium multicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium multicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | stable within | Penicillium multicolor |