Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacteroides thetaiotaomicron |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 80% soluble | Bacteroides thetaiotaomicron | 5737 | - |
membrane | 18% membrane associated | Bacteroides thetaiotaomicron | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
gel filtration | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Bacteroides thetaiotaomicron | branched polysaccharides | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | Bacteroides thetaiotaomicron | - |
maltotriose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Bacteroides thetaiotaomicron | maltooligosaccharides + maltose | - |
? | |
amylopectin + H2O | branched polysaccharides | Bacteroides thetaiotaomicron | maltooligosaccharides + maltose | - |
? | |
pullulan + H2O | - |
Bacteroides thetaiotaomicron | maltotriose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 77000, gel electrophoresis | Bacteroides thetaiotaomicron |
Synonyms | Comment | Organism |
---|---|---|
pullulanase type II | hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides | Bacteroides thetaiotaomicron |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacteroides thetaiotaomicron |