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Literature summary for 3.2.1.4 extracted from

  • Dos Santos, Y.Q.; de Veras, B.O.; de Franca, A.F.J.; Gorlach-Lira, K.; Velasques, J.; Migliolo, L.; Dos Santos, E.A.
    A new salt-tolerant thermostable cellulase from a marine Bacillus sp. strain (2018), J. Microbiol. Biotechnol., 28, 1078-1085 .
    View publication on PubMed
Search for more literature for 3.2.1.4

Application

Application Comment Organism
industry enzyme Bc22Cel is a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives Bacillus sp. SR22

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination, analysis, and comparisons, phylogenetic tree Bacillus sp. SR22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km and Vmax are 0.704 mg/ml and 0.02985 mmol/ml/min, respectively Bacillus sp. SR22

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Bacillus sp. SR22
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl the purified Bc22Cel shows a considerable halophilic property, being able to maintain more than 70% of residual activity when pre-incubated with 1.5 M NaCl for 1 h Bacillus sp. SR22

Organism

Organism UniProt Comment Textmining
Bacillus sp. SR22
-
 isolated from Siderastrea stellata tissue samples collected at Cabo Branco shore, Brasil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by ammonium sulfate fractionation and gel filtration Bacillus sp. SR22

Source Tissue

Source Tissue Comment Organism Textmining
cell culture growth of the strain SR22 marine isolate on CMC and sugarcane bagasse as sole carbon source, compared on solid media at different salinity ranges, overview Bacillus sp. SR22
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.02
-
 purified native enzyme, pH 6.5, 60°C Bacillus sp. SR22

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Bacillus sp. SR22 ?
-
 ?
sugarcane bagasse + H2O
-
 Bacillus sp. SR22 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, about, SDS-PAGE Bacillus sp. SR22

Synonyms

Synonyms Comment Organism
Bc22Cel
-
 Bacillus sp. SR22
endo-beta-glucanase
-
 Bacillus sp. SR22
endoglucanase
-
 Bacillus sp. SR22

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus sp. SR22

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Bacillus sp. SR22

General Information

General Information Comment Organism
physiological function endo-beta-glucanases (E.C. 3.2.1.4) are able to cleave the cellulosic structure in internal sites, generating a large number of ends where exo-beta-glucanases (E.C. 3.2.1.91 and EC 3.2.1.176) are able to act on degrading the reducing and non-reducing ends of cellulose to small glucose oligomers (cellobiose and cellodextrins), which in turn are hydrolyzed to single glucose units by enzymes denominated by beta-glycosidases (E.C. 3.2.1.21) Bacillus sp. SR22