Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
cellopentaose | pH 7.0, 37°C | uncultured bacterium | |
1.4 | - |
4-nitrophenyl D-cellobioside | pH 7.0, 37°C | uncultured bacterium | |
5.1 | - |
cellohexaose | pH 7.0, 37°C | uncultured bacterium | |
13.3 | - |
Barley beta-glucan | pH 7.0, 37°C | uncultured bacterium | |
18.3 | - |
cellotetraose | pH 7.0, 37°C | uncultured bacterium | |
20.8 | - |
carboxymethyl cellulose | pH 7.0, 37°C | uncultured bacterium | |
28.3 | - |
hydroxyethylcellulose | pH 7.0, 37°C | uncultured bacterium | |
47.3 | - |
Laminarin | pH 7.0, 37°C | uncultured bacterium | |
151.7 | - |
cellotriose | pH 7.0, 37°C | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | F4YTG7 | isolated from paddy soil | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl D-cellobioside + H2O | - |
uncultured bacterium | 4-nitrophenol + D-cellobiose | - |
? | |
barley beta-glucan + H2O | - |
uncultured bacterium | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
uncultured bacterium | D-glucose + ? | - |
? | |
cellohexaose + 5 H2O | - |
uncultured bacterium | 6 D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
uncultured bacterium | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
uncultured bacterium | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
uncultured bacterium | 3 D-glucose | - |
? | |
hydroxyethylcellulose + H2O | - |
uncultured bacterium | ? | - |
? | |
laminarin + H2O | - |
uncultured bacterium | 6 D-glucose | - |
? | |
additional information | Umcel9y-1 is an efficient endoglucanase and also exhibits high activities of exoglucanase and transglycosylation. The transglycosylation products of Umcel9y-1 including sophorose, laminaribiose, and gentiobiose, and transglycosylation are detected under all activated conditions. The decreasing order of catalytic efficiency for polysaccharides, cellooligosaccharides, and aryl-beta-glycosides is p-nitrophenyl-D-cellobioside, barley glucan, cellopentaose, cellotetraose, cellotriose, hydroxyethylcellulose, cellohexaose, laminarin, and carboxymethylcellulose, respectively | uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
umcel9y-1 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.7 | - |
cellohexaose | pH 7.0, 37°C | uncultured bacterium | |
42.3 | - |
cellopentaose | pH 7.0, 37°C | uncultured bacterium | |
49.2 | - |
carboxymethyl cellulose | pH 7.0, 37°C | uncultured bacterium | |
127.5 | - |
Laminarin | pH 7.0, 37°C | uncultured bacterium | |
159 | - |
cellotetraose | pH 7.0, 37°C | uncultured bacterium | |
185.6 | - |
hydroxyethylcellulose | pH 7.0, 37°C | uncultured bacterium | |
466 | - |
4-nitrophenyl D-cellobioside | pH 7.0, 37°C | uncultured bacterium | |
897 | - |
cellotriose | pH 7.0, 37°C | uncultured bacterium | |
3276 | - |
Barley beta-glucan | pH 7.0, 37°C | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
carboxymethyl cellulose | pH 7.0, 37°C | uncultured bacterium | |
2.7 | - |
Laminarin | pH 7.0, 37°C | uncultured bacterium | |
5.6 | - |
hydroxyethylcellulose | pH 7.0, 37°C | uncultured bacterium | |
5.9 | - |
cellotriose | pH 7.0, 37°C | uncultured bacterium | |
8.2 | - |
cellohexaose | pH 7.0, 37°C | uncultured bacterium | |
8.7 | - |
cellotetraose | pH 7.0, 37°C | uncultured bacterium | |
68.7 | - |
cellopentaose | pH 7.0, 37°C | uncultured bacterium | |
246 | - |
Barley beta-glucan | pH 7.0, 37°C | uncultured bacterium | |
337 | - |
4-nitrophenyl D-cellobioside | pH 7.0, 37°C | uncultured bacterium |