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Literature summary for 3.2.1.4 extracted from

  • Bendig, C.; Weuster-Botz, D.
    Reaction engineering analysis of cellulase production with Trichoderma reesei RUT-C30 with intermittent substrate supply (2013), Bioprocess Biosyst. Eng., 36, 893-900.
    View publication on PubMed

Application

Application Comment Organism
synthesis production of enzyme in parallel-operated shake flasks and, alternatively, in parallel-operated stirred-tank bioreactors on a 10-m. scale. Reaction conditions with 53.3 g/l microcrystalline cellulose in the initial medium, no lactose feeding and 3.3 g/l and day intermittent ammonium sulfate addition are optimal. The optimum substrate supply on a liter-scale results in the production of 4.88 filter paper units of enzyme per ml with after 96 h Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
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Trichoderma reesei RUT C-30
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