Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Penicillium oxalicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 38% residual activity at 5 mM | Penicillium oxalicum | |
Cu2+ | about 80% residual activity at 5 mM | Penicillium oxalicum | |
D-xylose | about 55% residual activity in the presence of 20 mM xylose | Penicillium oxalicum | |
EDTA | about 75% residual activity at 5 mM | Penicillium oxalicum | |
K+ | about 77% residual activity at 5 mM | Penicillium oxalicum | |
additional information | not inhibited by glucose, arabinose, mannose, and galactose | Penicillium oxalicum | |
Pb2+ | about 40% residual activity at 5 mM | Penicillium oxalicum | |
SDS | about 42% residual activity at 5 mM | Penicillium oxalicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.05 | - |
4-nitrophenyl beta-D-xylopyranoside | at pH 7.0 and 50°C | Penicillium oxalicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mn2+, Ca2+, Fe3+, Co2+ and Zn2+ have no effects on enzyme activity | Penicillium oxalicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium oxalicum | S7ZRR2 | - |
- |
Penicillium oxalicum 114-2 | S7ZRR2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Penicillium oxalicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Penicillium oxalicum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Penicillium oxalicum 114-2 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
additional information | no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-L-arabinofuranoside, 4-nitrophenyl-D-cellobioside, sugarcane bagasse xylan, corncob xylan, beechwood xylan, glucan, mannan, polygalactose and arabinan | Penicillium oxalicum | ? | - |
? | |
additional information | no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-L-arabinofuranoside, 4-nitrophenyl-D-cellobioside, sugarcane bagasse xylan, corncob xylan, beechwood xylan, glucan, mannan, polygalactose and arabinan | Penicillium oxalicum 114-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Penicillium oxalicum |
? | x * 38610, calculated from amino acid sequence | Penicillium oxalicum |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase | - |
Penicillium oxalicum |
xyl43 | - |
Penicillium oxalicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium oxalicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | more than 40% activity between 30 and 60°C | Penicillium oxalicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme remains stable up to 50°C after 30 min of incubation but the activity rapidly decreases when the temperature above 50°C | Penicillium oxalicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Penicillium oxalicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme retains 76.35% and 55.85% of catalytic activities at pH 6.0 and 9.0, respectively | Penicillium oxalicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | the enzyme is relatively stable in the range of pH 6.0 to 9.0, retaining more than 61% residual activity after 30 min of incubation | Penicillium oxalicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
28.09 | - |
D-xylose | at pH 7.0 and 50°C | Penicillium oxalicum |