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Literature summary for 3.2.1.37 extracted from

  • Li, T.; Wu, Y.; He, J.
    Heterologous expression, characterization and application of a new beta-xylosidase identified in solventogenic Clostridium sp. strain BOH3 (2018), Process Biochem., 67, 99-104 .
No PubMed abstract available
Search for more literature for 3.2.1.37

Activating Compound

Activating Compound Comment Organism Structure
SDS 118% activity at 1 mM Clostridium sp. BOH3
Tween 80 120% activity at 1 mM Clostridium sp. BOH3

Inhibitors

Inhibitors Comment Organism Structure
4-Aminobenzoic acid about 90% residual activity at 10 mM Clostridium sp. BOH3
Ag+ complete inhibition at 0.1 mM Clostridium sp. BOH3
EDTA about 45% residual activity at 10 mM Clostridium sp. BOH3
Fe2+ about 70% residual activity at 1 mM Clostridium sp. BOH3
Hg2+ complete inhibition at 0.1 mM Clostridium sp. BOH3
Zn2+ about 75% residual activity at 1 mM Clostridium sp. BOH3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.71
-
 4-nitrophenyl alpha-D-xylopyranoside at pH 6.0 and 40°C Clostridium sp. BOH3

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 148% activity at 1 mM Clostridium sp. BOH3
Co2+ 139% activity at 1 mM Clostridium sp. BOH3
Mn2+ 135% activity at 1 mM Clostridium sp. BOH3
additional information not influenced by Na+, K+ and Ni2+ Clostridium sp. BOH3
Zn2+ enzyme activity can be enhanced more than 1.63times by addition of 0.1 mM Zn2+ Clostridium sp. BOH3

Organism

Organism UniProt Comment Textmining
Clostridium sp. BOH3 A0A024A6V9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Clostridium sp. BOH3 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl alpha-L-arabinopyranoside + H2O 3.2% activity compared to 4-nitrophenyl alpha-D-xylopyranoside Clostridium sp. BOH3 4-nitrophenol + alpha-L-arabinose
-
 ?
beechwood xylan + H2O 1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside Clostridium sp. BOH3 ?
-
 ?
birchwood xylan + H2O 1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside Clostridium sp. BOH3 ?
-
 ?
xylobiose + H2O
-
 Clostridium sp. BOH3 2 D-xylose
-
 ?
xylopentaose + H2O
-
 Clostridium sp. BOH3 ?
-
 ?
xylotetraose + H2O
-
 Clostridium sp. BOH3 ?
-
 ?
xylotriose + H2O
-
 Clostridium sp. BOH3 xylobiose + D-xylose
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, calculated from amino acid sequence Clostridium sp. BOH3
? x * 80000, thioredoxin-tagged enzyme, SDS-PAGE Clostridium sp. BOH3

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
 Clostridium sp. BOH3
Bxyl
-
 Clostridium sp. BOH3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Clostridium sp. BOH3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme keeps almost 100% of its initial activity when incubating below 50°C for 2 h at pH 6.0 Clostridium sp. BOH3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6
-
 Clostridium sp. BOH3