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Literature summary for 3.2.1.37 extracted from

  • Xiong, J.S.; Balland-Vanney, M.; Xie, Z.P.; Schultze, M.; Kondorosi, A.; Kondorosi, E.; Staehelin, C.
    Molecular cloning of a bifunctional beta-xylosidase/alpha-L-arabinosidase from alfalfa roots: heterologous expression in Medicago truncatula and substrate specificity of the purified enzyme (2007), J. Exp. Bot., 58, 2799-2810.
    View publication on PubMed
Search for more literature for 3.2.1.37

Application

Application Comment Organism
biotechnology use of stable transgenic Medicago truncatula plants as an expression system for purification and characterization of proteins Medicago sativa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Medicago truncatula Medicago sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 4-nitrophenyl-beta-D-xylopyranoside 37°C Medicago sativa
0.94
-
 4-nitrophenyl-alpha-L-arabinofuranoside 37°C Medicago sativa
1.2
-
 4-nitrophenyl-alpha-L-arabinopyranoside 37°C Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Medicago sativa enzyme is implicated in cell wall turnover of arabinose and xylose ?
-
 ?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
 isoform Xyl1, concanavalin A-binding protein
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Medicago sativa
-
root
-
 Medicago sativa
-
root nodule
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.4
-
 37°C Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Medicago sativa 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Medicago sativa 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl-alpha-L-arabinopyranoside + H2O
-
 Medicago sativa 4-nitrophenol + alpha-L-arabinose
-
 ?
arabinoxylan + H2O source of substrate: sugar beet Medicago sativa D-xylose + L-arabinose
-
 ?
arabinoxylan + H2O source of substrate: wheat Medicago sativa xylose + arabinose
-
 ?
cell wall polysaccharide from alfalfa root + H2O
-
 Medicago sativa xylose + arabinose
-
 ?
additional information enzyme is implicated in cell wall turnover of arabinose and xylose Medicago sativa ?
-
 ?
additional information cleavage of beta-1,4-linked D-xylo-oligosaccharides and alpha-1,5-linked L-arabino-oligosaccharides. Xylan from oat spelt is not a substrate Medicago sativa ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.96
-
 4-nitrophenyl-beta-D-xylopyranoside 37°C Medicago sativa
1.75
-
 4-nitrophenyl-alpha-L-arabinopyranoside 37°C Medicago sativa
4.7
-
 4-nitrophenyl-alpha-L-arabinofuranoside 37°C Medicago sativa