Cloned (Comment) | Organism |
---|---|
gene xynB3, expression of soluble wild-type and mutant D15G, D128G, and E178G enzymes in Escherichia coli strain BL21(DE3) | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and mutant D15G, D128G, and E178G XynB3s, 22-30 mg/ml protein, 0.005 ml protein solution is mixed with 0.005 ml reservoir solution and 0.001 ml detergent solution, equilibration to 0.5 ml with reservoir solution, X-ray diffraction structure determination and preliminary analysis at 2.2-2.5 A resolution | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q09LX0 | i.e. Bacillus stearothermophilus, strain T-6, gene xynB3 | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble wild-type and mutant D15G, D128G, and E178G enzymes from Escherichia coli strain BL21(DE3) by heat treatment and gel filtration | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
beta-D-xylosidase | - |
Geobacillus stearothermophilus |
More | the enzyme belongs to the glycosyl hydrolase family 43, GH43 | Geobacillus stearothermophilus |
XynB3 | - |
Geobacillus stearothermophilus |