Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-xylose | competitive inhibition | Blastobotrys adeninivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
p-nitrophenyl beta-D-xylopyranoside | for beta-xylosidase 1 | Blastobotrys adeninivorans | |
0.26 | - |
p-nitrophenyl beta-D-xylopyranoside | for beta-xylosidase 2 | Blastobotrys adeninivorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Blastobotrys adeninivorans | 5618 | - |
extracellular | - |
Blastobotrys adeninivorans | - |
- |
intracellular | - |
Blastobotrys adeninivorans | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
extra- and intracellular activity of beta-xylosidase-1, gel filtration, SDS-PAGE | Blastobotrys adeninivorans |
160000 | - |
beta-xylosidase-2, gel filtration, SDS-PAGE | Blastobotrys adeninivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylo-oligosaccharides + H2O | Blastobotrys adeninivorans | - |
beta-D-xylose + ? | - |
? | |
xylobiose + H2O | Blastobotrys adeninivorans | - |
beta-D-xylose + D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastobotrys adeninivorans | - |
yeast | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | active enzyme after deglycosylation by endoglycosidase H | Blastobotrys adeninivorans |
Purification (Comment) | Organism |
---|---|
to homogeneity by chromatography steps, separation of beta-xylosidase 1 and beta-xylosidase 2 | Blastobotrys adeninivorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | synthesis of enzyme only in xylose or methyl-beta-D-xylopyranoside media | Blastobotrys adeninivorans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.1 | - |
extracellular beta-xylosidase 2 | Blastobotrys adeninivorans |
21.8 | - |
intracellular beta-xylosidase 2 | Blastobotrys adeninivorans |
23.7 | - |
cell-wall bound enzyme | Blastobotrys adeninivorans |
26.6 | - |
intracellular beta-xylosidase 1 | Blastobotrys adeninivorans |
27.1 | - |
extracellular beta-xylosidase 1 | Blastobotrys adeninivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl-beta-D-xyloside + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + methanol | - |
? | |
p-nitrophenyl beta-D-xylopyranoside + H2O | high activity | Blastobotrys adeninivorans | p-nitrophenol + beta-D-xylopyranose | - |
? | |
xylo-oligosaccharides + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + ? | - |
? | |
xylobiose + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000-75000, beta-xylosidase-2, SDS-PAGE | Blastobotrys adeninivorans |
monomer | 1 * 60000-75000, beta-xylosidase-1, SDS-PAGE, gel filtration | Blastobotrys adeninivorans |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase-1 | - |
Blastobotrys adeninivorans |
beta-xylosidase-2 | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 64 | - |
Blastobotrys adeninivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | 5.2 | - |
Blastobotrys adeninivorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.1 | 6.4 | 50% activity at pH 4.1 and 6.4 | Blastobotrys adeninivorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
more stable than at pH 4.1 | Blastobotrys adeninivorans |