Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermoanaerobacterium saccharolyticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE, calculation from sequence of cDNA | Thermoanaerobacterium saccharolyticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylo-oligosaccharides + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + ? | - |
? | |
xylobiose + H2O | Thermoanaerobacterium saccharolyticum | - |
beta-D-xylose + D-xylose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacterium saccharolyticum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by heat treatment and 2step chromatography techniques | Thermoanaerobacterium saccharolyticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.53 | - |
- |
Thermoanaerobacterium saccharolyticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
o-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermoanaerobacterium saccharolyticum | o-nitrophenol + beta-D-xylopyranose | - |
? | |
p-nitrophenyl beta-D-xylopyranoside + H2O | high specificity, transxylosylation activity | Thermoanaerobacterium saccharolyticum | p-nitrophenol + beta-D-xylopyranose | - |
? | |
xylo-oligosaccharides + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
xylobiose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + D-xylose | - |
? | |
xylopentaose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + ? | - |
? | |
xylotetraose + H2O | - |
Thermoanaerobacterium saccharolyticum | xylotriose + beta-D-xylose | - |
? | |
xylotriose + H2O | - |
Thermoanaerobacterium saccharolyticum | beta-D-xylose + xylobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
stable up to, loss of activity at 85°C | Thermoanaerobacterium saccharolyticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.6 | high activity within | Thermoanaerobacterium saccharolyticum |