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Literature summary for 3.2.1.31 extracted from
- exo-beta-D-Glucuronidase from the fungus Kobayasia nipponica (1984), J. Biochem., 96, 1789-1797.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Protubera nipponica |  |
| D-saccharic acid 1,4-lactone | - |
Protubera nipponica | |
| delta-D-glucuronolactone | - |
Protubera nipponica | |
| heparin | - |
Protubera nipponica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | 2.9 | p-nitrophenyl-beta-D-glucuronide | - |
Protubera nipponica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration, SDS-PAGE | Protubera nipponica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Protubera nipponica | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Protubera nipponica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 forms | Protubera nipponica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100 | 533 | - |
Protubera nipponica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucuronide + H2O | - |
Protubera nipponica | 4-nitrophenol + D-glucuronic acid | - |
? | |
| additional information | - |
Protubera nipponica | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
10 min | Protubera nipponica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
form III | Protubera nipponica |
| 3.2 | - |
form II | Protubera nipponica |
| 3.4 | - |
form I | Protubera nipponica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 7 | form III | Protubera nipponica |
| 5 | 7 | form I | Protubera nipponica |
| 5.4 | 7 | form II | Protubera nipponica |
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