Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 108% of inital activity | Mycothermus thermophilus | |
Mg2+ | 1 mM, 127% of inital activity | Mycothermus thermophilus | |
Mn2+ | 1 mM, 105% of inital activity | Mycothermus thermophilus | |
Zn2+ | 1 mM, 121% of inital activity | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
starch | Km value 0.21 mg/ml, pH 5.5, 65°C | Mycothermus thermophilus | |
additional information | - |
maltose | Km value 3.9 mg/ml, pH 5.5, 65°C | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content 9.8% | Mycothermus thermophilus |
Purification (Comment) | Organism |
---|---|
- |
Mycothermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
89 | - |
pH 5.5, 65°C | Mycothermus thermophilus |
Storage Stability | Organism |
---|---|
4°C, pH 7.0 or 5.5, stable for 8 days | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Mycothermus thermophilus | ? | - |
? | |
amylose + H2O | - |
Mycothermus thermophilus | D-glucose + ? | - |
? | |
glycogen + H2O | - |
Mycothermus thermophilus | ? | - |
? | |
maltose + H2O | - |
Mycothermus thermophilus | 2 D-glucose | - |
? | |
starch + H2O | - |
Mycothermus thermophilus | D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 *75000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
substrate maltose | Mycothermus thermophilus |
70 | - |
substrate starch | Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable for 1 h | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Mycothermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycothermus thermophilus | isoelectric focusing | - |
8.3 |