Application | Comment | Organism |
---|---|---|
synthesis | production of high levels of cell extract and extracellular invertases when grown under submerged fermentation and solid-state fermentation, using agroindustrial products or residues as substrates, mainly soy bran and wheat bran, at 40°C for 72 h and 96 h, respectively. Addition of glucose or fructose in submerged fermentation inhibits enzyme production, while the addition of 1% (w/v) peptone as organic nitrogen source enhances the production by 3.7fold. 1% (w/v) (NH4)2HPO4 inhibits enzyme production around 80% | Paecilomyces variotii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
sucrose | extracellular enzyme, pH 4.5, 60°C | Paecilomyces variotii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paecilomyces variotii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM enhances the extracellular activity about 154%, and enhances the intracellular activity about 202% | Paecilomyces variotii | |
Mg2+ | 1 mM enhances the extracellular activity about 180% | Paecilomyces variotii | |
Mn2+ | 1 mM enhances the intracellular activity about 317% | Paecilomyces variotii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
210000 | - |
1 * 210000, SDS-PAGE of extracellular enzyme | Paecilomyces variotii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paecilomyces variotii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content of 53.6%, extracellular enzyme | Paecilomyces variotii |
Purification (Comment) | Organism |
---|---|
extracellular isoform | Paecilomyces variotii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
601 | - |
extracellular enzyme, pH 4.5, 60°C | Paecilomyces variotii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Paecilomyces variotii | alpha-D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 210000, SDS-PAGE of extracellular enzyme | Paecilomyces variotii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
both extracellular and cell extract enzyme | Paecilomyces variotii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h, both extracellular and cell extract enzyme | Paecilomyces variotii |
70 | - |
half-life 10 min, both extracellular and cell extract enzyme | Paecilomyces variotii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | both extracellular and intracellular enzyme | Paecilomyces variotii |