General Stability | Organism |
---|---|
enzyme activity almost constant within investigated time range of 250 min at room temperature | Helianthus tuberosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, 37% inhibition; 1 mM, 88% inhibition | Helianthus tuberosus | |
CuSO4 | 0.1 mM, 46% inhibition; 1 mM, 100% inhibition | Helianthus tuberosus | |
fructose | 40 mM, 52% inhibition; 4 mM, 22% inhibition | Helianthus tuberosus | |
glucose | 40 mM, 100% inhibition | Helianthus tuberosus | |
HgCl2 | 0.1 mM, 48% inhibition; 1 mM, 89% inhibition | Helianthus tuberosus | |
additional information | MgCl2 and CaCl2 (1 mM) are typical alkaline invertase inhibitors, they do not inhibit the neutral invertase | Helianthus tuberosus | |
Tris | 10 mM, 87% inhibition; 1 mM, 74% inhibition | Helianthus tuberosus | |
ZnCl2 | 0.1 mM, 84% inhibition; 1 mM, 100% inhibition | Helianthus tuberosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21 | - |
sucrose | 37°C | Helianthus tuberosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial matrix | highest invertase activities compared to inner or outer membrane, since transport capacity through matrix is limiting enzyme activity, enzyme must be located on inner matrix | Helianthus tuberosus | 5759 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Helianthus tuberosus | - |
D-fructose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | - |
Jerusalem artichoke | - |
Purification (Comment) | Organism |
---|---|
mitochondrial fraction isolated by several centrifugation steps from filtrate (cheesecloth) of ground and homogenized tubers, final centrifugation step with mannitol gradient (lower fraction excluding the bottom) followed by several washing steps of the pellet, resuspension in 10 mM morpholinepropanesulfonic acid + 70 mM mannitol buffer, pH 7.4, further centrifugation steps to separate mitoplast pellet from intermembrane space and outer membrane and separate the mitoplast pellet after repeated freeze-thawing rupture into matrix supernatant and inner membrane pellet, washed and resuspended in 10 mM morpholinepropanesulfonic acid buffer, pH 7.4 | Helianthus tuberosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tuber | - |
Helianthus tuberosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0005 | - |
inner membrane | Helianthus tuberosus |
0.0008 | - |
outer membrane | Helianthus tuberosus |
0.0116 | - |
matrix, 10 min, room temperature, control | Helianthus tuberosus |
0.014 | - |
untreated mitochondria, 37°C | Helianthus tuberosus |
0.0196 | - |
matrix | Helianthus tuberosus |
0.0231 | - |
mitochondria, alamethicin treated to enable unrestricted transport, 37°C | Helianthus tuberosus |
0.0269 | - |
matrix, 10 min, 37°C | Helianthus tuberosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Helianthus tuberosus | D-fructose + D-glucose | - |
? | |
sucrose + H2O | 100 mM sucrose, 1 mg/ml mitochondria, room temperature (22-25°C), 300 mM mannitol + 10 mM morpholinepropanesulfonic acid buffer, pH 7.4, no enzyme activity with maltose or raffinose | Helianthus tuberosus | D-fructose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neutral invertase | - |
Helianthus tuberosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
pH adjusted with KOH or HCl | Helianthus tuberosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | less than 20% activity at pH 5, more than 80% activity between pH 6.2 and 7.7 with maximum at pH 7 and 7.2, 50-70% activity between pH 8 and 8.5 | Helianthus tuberosus |