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Literature summary for 3.2.1.24 extracted from

  • Tulsiani, D.R.P.; Opheim, D.J.; Touster, O.
    Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes (1977), J. Biol. Chem., 252, 3227-3233.
    View publication on PubMed
Search for more literature for 3.2.1.24

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation up to 2.5 mM Rattus sp.
ATP 25% activation at 1 mM Rattus sp.
bovine serum albumin 35% activation at 0.125 mg/ml Rattus sp.
CMP 25% activation at 1 mM Rattus sp.
dithiothreitol nearly 60% activation up to 2.5 mM Rattus sp.
L-fucose 20% activation at 250 mM Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-mannopyranose 30% inhibition at 250 mM Rattus sp.
Cu2+ potent inhibitor Rattus sp.
Fe2+ potent inhibitor Rattus sp.
p-chloromercuribenzenesulfonic acid strong inhibition at 1 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
30
-
 p-nitrophenyl-alpha-D-mannopyranoside
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Rattus sp. 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 60% activation up to 2.5 mM Rattus sp.
Zn2+ 30% activation up to 2.5 mM Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 4 * 75000, denaturating electrophoresis Rattus sp.
295000
-
 gel filtration Rattus sp.

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1
-
-
 Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 75000, denaturating electrophoresis Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rattus sp.