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Literature summary for 3.2.1.23 extracted from

  • Matsuzawa, T.; Watanabe, M.; Kameda, T.; Kameyama, A.; Yaoi, K.
    Cooperation between beta-galactosidase and an isoprimeverose-producing oligoxyloglucan hydrolase is key for xyloglucan degradation in Aspergillus oryzae (2019), FEBS J., 286, 3182-3193 .
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.42
-
 4-nitrophenyl beta-D-galactopyranoside pH 4.5, 60°C Aspergillus oryzae
1.76
-
 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
2.41
-
 beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
104
-
 lactose pH 4.5, 60°C Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
122000
-
 SDS-PAGE, recombinant glycosylated enzyme Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xyloglucan oligosaccharide + H2O Aspergillus oryzae the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex galactopyranose + xyloglucan oligosaccharide
-
 ?
xyloglucan oligosaccharide + H2O Aspergillus oryzae ATCC 42149 the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex galactopyranose + xyloglucan oligosaccharide
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae Q2UCU3
-
-
Aspergillus oryzae ATCC 42149 Q2UCU3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant LacA is N-glycosylated and the protein band is shifted from approximately 122000 Da to 108000 Da by endoglycosidase Hf treatment Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3 3.9 substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C Aspergillus oryzae
17.7
-
 substrate: lactose, pH 4.5, 60°C Aspergillus oryzae
35.8
-
 substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C Aspergillus oryzae
71
-
 substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C Aspergillus oryzae
121
-
 substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C Aspergillus oryzae
299
-
 substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenol + beta-D-galactopyranose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Aspergillus oryzae ATCC 42149 4-nitrophenol + beta-D-galactopyranose
-
 ?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
-
 Aspergillus oryzae alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
 ?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
-
 Aspergillus oryzae ATCC 42149 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
 ?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
-
 Aspergillus oryzae alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
 ?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + H2O
-
 Aspergillus oryzae alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
 ?
beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + H2O
-
 Aspergillus oryzae alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + beta-D-galactose
-
 ?
lactose + H2O
-
 Aspergillus oryzae D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Aspergillus oryzae ATCC 42149 D-glucose + D-galactose
-
 ?
xyloglucan oligosaccharide + H2O the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex Aspergillus oryzae galactopyranose + xyloglucan oligosaccharide
-
 ?
xyloglucan oligosaccharide + H2O the enzyme cleaves galactopyranosyl residues from xyloglucan oligosaccharides, but not from xyloglucan polysaccharide. An isoprimeverose unit at the nonreducing end of the main chain of xyloglucan oligosaccharides blocks LacA activity Aspergillus oryzae galactopyranose + xyloglucan oligosaccharide
-
 ?
xyloglucan oligosaccharide + H2O the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA-xyloglucan oligosaccharide complex Aspergillus oryzae ATCC 42149 galactopyranose + xyloglucan oligosaccharide
-
 ?
xyloglucan oligosaccharide + H2O the enzyme cleaves galactopyranosyl residues from xyloglucan oligosaccharides, but not from xyloglucan polysaccharide. An isoprimeverose unit at the nonreducing end of the main chain of xyloglucan oligosaccharides blocks LacA activity Aspergillus oryzae ATCC 42149 galactopyranose + xyloglucan oligosaccharide
-
 ?

Synonyms

Synonyms Comment Organism
LacA
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
65
-
 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
289
-
 beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
525
-
 4-nitrophenyl beta-D-galactopyranoside pH 4.5, 60°C Aspergillus oryzae
869
-
 lactose pH 4.5, 60°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Aspergillus oryzae

Expression

Organism Comment Expression
Aspergillus oryzae expression of the lacA gene is repressed by D-glucose down
Aspergillus oryzae induced in the presence of xyloglucan oligosaccharides up

General Information

General Information Comment Organism
physiological function the combinations of beta-galactosidase LacA and isoprimeverose-producing oligoxyloglucan hydrolase IpeA is crucial for xyloglucan degradation. Specifically, the combination of LacA and IpeA is a key step in the degradation of xyloglucan oligosaccharides, because IpeA requires LacA for the removal of galactopyranosyl residues, which prevent IpeA activity, and LacA requires IpeA for the removal of isoprimeverose units, which interfere with the formation of the LacA -xyloglucan oligosaccharide complex Aspergillus oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.36
-
 lactose pH 4.5, 60°C Aspergillus oryzae
37
-
 alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
120
-
 beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc pH 4.5, 60°C Aspergillus oryzae
370
-
 4-nitrophenyl beta-D-galactopyranoside pH 4.5, 60°C Aspergillus oryzae