KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Irpex lacteus | |
19.9 | - |
beta(1->3)-galactotriose | - |
Irpex lacteus | |
31.6 | - |
beta-(1->3)-galactobiose | - |
Irpex lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Irpex lacteus |
51000 | - |
1 * 51000, denaturing PAGE | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | - |
i.e. Polyporus tulipiferae | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Irpex lacteus |
Purification (Comment) | Organism |
---|---|
- |
Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-(1->3)-D-galactan + H2O | - |
Irpex lacteus | beta-D-galactose + ? | - |
? | |
beta-1,3-galactobiose + H2O | - |
Irpex lacteus | beta-D-galactose | - |
? | |
beta-1,3-galactotriose + H2O | - |
Irpex lacteus | ? | - |
? | |
methyl beta-D-galactopentaoside + H2O | - |
Irpex lacteus | ? | - |
? | |
methyl beta-D-galactotetraoside + H2O | - |
Irpex lacteus | ? | - |
? | |
additional information | specifically hydrolyzes oligosaccharides and polymers of (1-3)-linked beta-D-galactopyranosyl residues. Hydrolysis of carbohydrate portion of acacia gum and radish arabinogalactan-proteins increases with their decreasing branching. The action pattern of the enzyme includes the capability of bypassing the branching points. The side chains carrying additional D-galactosyl group at the reducing termini are released as neutral (1-6)-linked beta-D-galactooligosaccharides and their acidic derivatives having a 4-O-methyl-beta-D-glucuronosyl residue as the nonreducing end-group | Irpex lacteus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 51000, denaturing PAGE | Irpex lacteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
pH 4.6, 10 min, stable up to | Irpex lacteus |
60 | - |
pH 4.6, 10 min, complete inactivation | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
hydrolysis of beta-(1,3)-D-galactan | Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | 4°C, 24 h, stable | Irpex lacteus |