Protein Variants | Comment | Organism |
---|---|---|
F328A | transglycosylation is markedly increased compared to wild-type enzyme. kcat/Km value is more than twofold lower than for the wild type enzyme. 16-fold higher yield of products with the (alpha1,2)-bond as compared to products produced by the wild type enzyme | Thermotoga maritima |
G385L | transglycosylation is markedly increased compared to wild-type enzyme. Mutation G385L results in a twofold decrease in the specific activity of the enzyme during its purification. The kinetic parameters are not determined for the mutant G385L due to its instability | Thermotoga maritima |
L195C | kcat/Km value is more than twofold lower than for the wild type enzyme | Thermotoga maritima |
P402D | transglycosylation is markedly increased compared to wild-type enzyme. kcat/Km value is more than twofold lower than for the wild type enzyme. 4-fold higher yield of products with the (alpha1,2)-bond as compared to products produced by the wild type enzyme | Thermotoga maritima |
P402S | no significant changes in the kinetic parameters of hydrolysis | Thermotoga maritima |
W85Y | no significant changes in the kinetic parameters of hydrolysis | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme 328A | Thermotoga maritima | |
0.1 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme F194K | Thermotoga maritima | |
0.11 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, wild-type enzyme | Thermotoga maritima | |
0.11 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme G385L | Thermotoga maritima | |
0.11 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402S | Thermotoga maritima | |
0.32 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme L195C | Thermotoga maritima | |
0.43 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402D | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | G4FEF4 | - |
- |
Thermotoga maritima DSM 3109 | G4FEF4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | hydrolysis is accompanied by transglycosylation resulting in production of a mixture of (alpha1,2)-, (alpha1,3)-, and (alpha1,6)-4-nitrophenyl-digalactosides | Thermotoga maritima | 4-nitrophenol + D-galactopyranose | - |
? | |
4-nitrophenyl alpha-D-galactopyranoside + H2O | hydrolysis is accompanied by transglycosylation resulting in production of a mixture of (alpha1,2)-, (alpha1,3)-, and (alpha1,6)-4-nitrophenyl-digalactosides | Thermotoga maritima DSM 3109 | 4-nitrophenol + D-galactopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, wild type enzyme and its mutants W85Y, F194K, L195C, F328A, P402D, P402S, and G385L, SDS-PAGE | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme 328A | Thermotoga maritima | |
8 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, wild-type enzyme | Thermotoga maritima | |
8.15 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme G385L | Thermotoga maritima | |
9.8 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402S | Thermotoga maritima | |
10.73 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402D | Thermotoga maritima | |
12.2 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme L195C | Thermotoga maritima | |
20.4 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme F194K | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.57 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme 328A | Thermotoga maritima | |
24.95 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402D | Thermotoga maritima | |
38.76 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme L195C | Thermotoga maritima | |
72.07 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, wild-type enzyme | Thermotoga maritima | |
74.09 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme G385L | Thermotoga maritima | |
88.28 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme P402S | Thermotoga maritima | |
198.06 | - |
4-nitrophenyl alpha-D-galactopyranoside | 37°C, pH 5.0, mutant enzyme F194K | Thermotoga maritima |