Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 10 mM, complete inactivation | Thermomyces lanuginosus | |
Ca2+ | - |
Thermomyces lanuginosus | |
Hg2+ | - |
Thermomyces lanuginosus | |
Zn2+ | - |
Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.13 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
1.17 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
1.61 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Thermomyces lanuginosus CBS 395.62/b | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content 5.3% | Thermomyces lanuginosus |
Purification (Comment) | Organism |
---|---|
- |
Thermomyces lanuginosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Thermomyces lanuginosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1813 | - |
pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus |
Storage Stability | Organism |
---|---|
-20°C, 4-5 days, 60-70% loss of activity | Thermomyces lanuginosus |
4°C, 4-5 days, 60-70% loss of activity | Thermomyces lanuginosus |
highly stable in freeze-dried form or in buffered solutions containing 10-20% (w/v) NaCl | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus | 4-nitrophenol + alpha-D-galactose | - |
? | |
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | 4-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus | D-galactose + sucrose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | D-galactose + sucrose | - |
? | |
stachyose + H2O | - |
Thermomyces lanuginosus | raffinose + D-galactose | - |
? | |
stachyose + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | raffinose + D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 93000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 6.4-8.3, stable for at least 24 h | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3914 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
6946 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
7659 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.4 | 8.3 | 55°C, stable for at least 24 h | Thermomyces lanuginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces lanuginosus | isoelectric focusing | - |
3.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3455 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
4305 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
6555 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus |