Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 0.25 M | Formosa algae | |
Zn2+ | 0.25 M | Formosa algae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 0.25 M, activates | Formosa algae | |
Ca2+ | 0.25 M, activates | Formosa algae | |
Mg2+ | 0.25 M, activates | Formosa algae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Formosa algae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Formosa algae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract is detected after 4 h of bacterial growth, and maximal fucoidanase activity is observed after 12 h of growth | Formosa algae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fucoidan + H2O | the enzyme catalyzes the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase does not hydrolyze carrageenan. Desulfated fucoidan from Fucus evanescens is hydrolysed very weakly in contrast to deacetylated fucoidan, which is hydrolysed more actively compared to the native fucoidan from Fucus evanescens. Analysis of the structure of the enzymatic products shows that the enzyme shows an endo-type action that is specific for (1->4)-bonds in a polysaccharide molecule built up of alternating 3- and 4-linked alpha-L-fucopyranose residues sulfated mainly at position 2 | Formosa algae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Formosa algae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
60 min, activity of fucoidanase is considerably reduced | Formosa algae |
55 | - |
40 min, complete inactivation | Formosa algae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.1 | - |
Formosa algae |