Cloned (Comment) | Organism |
---|---|
- |
Apis cerana japonica |
DNA and amino acid sequence determination and anaylsis | Apis cerana japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | alpha-glucosidase I shows negative cooperative behaviour with substrates sucrose, maltose, and 4-nitrophenyl-alpha-D-glucopyranoside, but positive cooperativity with substrate turanose | Apis cerana japonica | |
0.21 | - |
p-nitrophenyl alpha-D-glucoside | - |
Apis cerana japonica | |
0.21 | - |
4-nitrophenyl-alpha-D-glucopyranoside | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
0.4 | - |
2-((2-(guanin-9-yl)ethyl)(2-((2-hydroxyethyl)((2-phosphonoethyl)amino)ethyl)amino)ethyl)phosphonic acid | - |
Apis cerana japonica | |
0.4 | - |
maltotriose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
1.3 | - |
maltotetraose | - |
Apis cerana japonica | |
1.3 | - |
maltotetraose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
1.5 | - |
maltose | - |
Apis cerana japonica | |
1.5 | - |
maltose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
2.4 | - |
sucrose | - |
Apis cerana japonica | |
5.5 | - |
maltopentaose | - |
Apis cerana japonica | |
5.5 | - |
maltopentaose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
8.7 | - |
kojibiose | - |
Apis cerana japonica | |
8.7 | - |
kojibiose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66515 | - |
1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis | Apis cerana japonica |
74000 | - |
1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis | Apis cerana japonica |
82000 | - |
alpha-glucosidase I, native PAGE | Apis cerana japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apis cerana japonica | A1IHL0 | - |
- |
Apis cerana japonica | A1IHL0 | alpha-glucosidase I; Japanese honeybee | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | alpha-glucosidase I contains 15% carbohydrate | Apis cerana japonica |
Purification (Comment) | Organism |
---|---|
by salting-out chromatography and gel filtration | Apis cerana japonica |
native alpha-glucosidase I by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration | Apis cerana japonica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
crude extract | Apis cerana japonica |
13 | - |
purified enzyme | Apis cerana japonica |
13 | - |
purified alpha-glucosidase I | Apis cerana japonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Apis cerana japonica | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucoside + H2O | - |
Apis cerana japonica | 4-nitrophenol + D-glucose | - |
? | |
kojibiose + H2O | - |
Apis cerana japonica | alpha-D-glucose + D-glucose | - |
? | |
kojibiose + H2O | - |
Apis cerana japonica | ? | - |
? | |
maltopentaose + H2O | - |
Apis cerana japonica | ? | - |
? | |
maltopentaose + H2O | - |
Apis cerana japonica | alpha-D-glucose + ? | - |
? | |
maltose + H2O | - |
Apis cerana japonica | ? | - |
? | |
maltose + H2O | - |
Apis cerana japonica | alpha-D-glucose + D-glucose | - |
? | |
maltotetraose + H2O | - |
Apis cerana japonica | ? | - |
? | |
maltotetraose + H2O | - |
Apis cerana japonica | alpha-D-glucose | - |
? | |
maltotriose + H2O | - |
Apis cerana japonica | ? | - |
? | |
maltotriose + H2O | - |
Apis cerana japonica | maltose + alpha-D-glucose | - |
? | |
additional information | no activity toward isomaltose, nigerose or soluble starch | Apis cerana japonica | ? | - |
? | |
additional information | substrate specificity, overview, the enzyme is also active with sucrose and turanose | Apis cerana japonica | ? | - |
? | |
sucrose + H2O | - |
Apis cerana japonica | D-fructose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 82000, alpha-glucosidase I, SDS-PAGE | Apis cerana japonica |
monomer | 1 * 82000, SDS-PAGE, 1 * 74000, SDS-PAGE, deglycosylated enzyme, 1 * 66515, sequence analysis | Apis cerana japonica |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucosidase I | - |
Apis cerana japonica |
alpha-glucoside glucohydrolase | - |
Apis cerana japonica |
JHGase I | - |
Apis cerana japonica |
maltase | - |
Apis cerana japonica |
More | the enzyme belongs to the alpha-amylase enzyme family | Apis cerana japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Apis cerana japonica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Apis cerana japonica |
40 | - |
purified alpha-glucosidase I, 15 min, stable up to | Apis cerana japonica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
kojibiose | - |
Apis cerana japonica | |
8.7 | - |
kojibiose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
13 | - |
maltotetraose | - |
Apis cerana japonica | |
13 | - |
p-nitrophenyl alpha-D-glucoside | - |
Apis cerana japonica | |
13 | - |
maltotriose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
13 | - |
maltopentaose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
13 | - |
4-nitrophenyl-alpha-D-glucopyranoside | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
15 | - |
maltotriose | - |
Apis cerana japonica | |
15 | - |
maltopentaose | - |
Apis cerana japonica | |
15 | - |
maltotetraose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
22 | - |
maltose | - |
Apis cerana japonica | |
22 | - |
maltose | pH 5.0, 37°C, alpha-glucosidase I | Apis cerana japonica | |
40 | - |
sucrose | - |
Apis cerana japonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Apis cerana japonica |
5 | - |
alpha-glucosidase I | Apis cerana japonica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | - |
Apis cerana japonica |
4.5 | 10.5 | purified alpha-glucosidase I, 24 h, 4°C, stable within this range | Apis cerana japonica |