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Literature summary for 3.2.1.195 extracted from

  • Yu, H.; Liu, Q.; Zhang, C.; Lu, M.; Fu, Y.; Im, W.-T.; Jin, F.
    A new ginsenosidase from Aspergillus strain hydrolyzing 20-O-multi-glycoside of PPD ginsenoside (2009), Proc. Biochem., 44, 772-775.
No PubMed abstract available
Search for more literature for 3.2.1.195

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Aspergillus sp.
Pb2+
-
 Aspergillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 50–100 mM slightly enhances the ginsenosidase type II activity Aspergillus sp.
Mg2+ 50–100 mM slightly enhances the ginsenosidase type II activity Aspergillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Aspergillus sp.

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Aspergillus sp. g48p
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ginsenoside Rb1 + H2O the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside Aspergillus sp. ginsenoside Rd + D-glucose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rb1 + H2O the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside Aspergillus sp. g48p ginsenoside Rd + D-glucose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rb2 + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinopyranoside Aspergillus sp. ginsenoside Rd + L-arabinopyranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rb2 + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinopyranoside Aspergillus sp. g48p ginsenoside Rd + L-arabinopyranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rb3 + H2O the enzyme hydrolyzes the 20-O-beta-(1->6)-xyloside Aspergillus sp. ginsenoside Rd + D-xylose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rb3 + H2O the enzyme hydrolyzes the 20-O-beta-(1->6)-xyloside Aspergillus sp. g48p ginsenoside Rd + D-xylose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rc + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinofuranoside Aspergillus sp. ginsenoside Rd + L-arabinofuranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rc + H2O the enzyme hydrolyzes the 20-O-alpha-(1->6)-arabinofuranoside Aspergillus sp. g48p ginsenoside Rd + L-arabinofuranose the enzyme produces mainly ginsenoside Rd, and small amount of ginsenoside 20(S)-Rg3 ?
ginsenoside Rd + H2O slowly hydrolyzes the 20-O-glucoside of Rd to produce very small amounts of ginsenoside Rg3 Aspergillus sp. ginsenoside Rg3 + D-glucose
-
 ?
additional information ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. ?
-
 ?
additional information ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1 Aspergillus sp. g48p ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Aspergillus sp.

Synonyms

Synonyms Comment Organism
ginsenosidase type II ambiguous Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus sp.
50
-
-
 Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus sp.