BRENDA - Enzyme Database
show all sequences of 3.2.1.188

Rüdiger, W.: Avenacosidase from oat: purification, sequence analysis and biochemical characterization of a new member of the BGA family of beta-glucosidases

Gus-Mayer, S.; Brunner, H.; Schneider-Poetsch, H.A.; Plant Mol. Biol. 26, 909-921 (1994) View publication on PubMed

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
etioplast
-
Avena sativa
9513
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
5 or 6 * 60000, SDS-PAGE
Avena sativa
300000
350000
gel filtration
Avena sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
avenacoside A + H2O
Avena sativa
-
26-desgluco-avenacoside A + D-glucose
-
-
?
avenacoside B + H2O
Avena sativa
-
26-desgluco-avenacoside B + D-glucose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Avena sativa
-
cultivar Pirol
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography, and Biogel A1.5 gel filtration
Avena sativa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
caryopsis
-
Avena sativa
-
coleoptile
-
Avena sativa
-
leaf
primary leaves
Avena sativa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.72
-
purified enzyme, using 4-nitrophenyl-beta-D-glucoside as substrate at pH 5.4 and 34°C
Avena sativa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3-nitrophenyl beta-D-galactopyranoside + H2O
-
723436
Avena sativa
3-nitrophenol + beta-D-galactopyranose
-
-
-
?
3-nitrophenyl beta-D-glucopyranoside + H2O
-
723436
Avena sativa
3-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
highest activity
723436
Avena sativa
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
723436
Avena sativa
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
-
723436
Avena sativa
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
lowest activity
723436
Avena sativa
4-nitrophenol + D-xylose
-
-
-
?
avenacoside A + H2O
-
723436
Avena sativa
26-desgluco-avenacoside A + D-glucose
-
-
-
?
avenacoside B + H2O
-
723436
Avena sativa
26-desgluco-avenacoside B + D-glucose
-
-
-
?
additional information
no activity with alpha-glycosides, beta-thioglucoside,cellobiose (beta1-4), gentiobiose (beta 1-6), laminaribiose (beta1-3), alpha-sophorose (beta1-2), 4-nitrophenyl-beta-D-xyloside, 3-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-galactoside, and 4-nitrophenyl-1-thio-beta-D-glucoside
723436
Avena sativa
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
pentamer or hexamer
5 or 6 * 60000, SDS-PAGE
Avena sativa
Synonyms
Synonyms
Commentary
Organism
26-desgluco-avenacosidase
-
Avena sativa
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
dissociation by freezing/thawing leads to complete loss of enzyme activity
Avena sativa
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
etioplast
-
Avena sativa
9513
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
5 or 6 * 60000, SDS-PAGE
Avena sativa
300000
350000
gel filtration
Avena sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
avenacoside A + H2O
Avena sativa
-
26-desgluco-avenacoside A + D-glucose
-
-
?
avenacoside B + H2O
Avena sativa
-
26-desgluco-avenacoside B + D-glucose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, hydroxyapatite column chromatography, and Biogel A1.5 gel filtration
Avena sativa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
caryopsis
-
Avena sativa
-
coleoptile
-
Avena sativa
-
leaf
primary leaves
Avena sativa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.72
-
purified enzyme, using 4-nitrophenyl-beta-D-glucoside as substrate at pH 5.4 and 34°C
Avena sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3-nitrophenyl beta-D-galactopyranoside + H2O
-
723436
Avena sativa
3-nitrophenol + beta-D-galactopyranose
-
-
-
?
3-nitrophenyl beta-D-glucopyranoside + H2O
-
723436
Avena sativa
3-nitrophenol + D-glucopyranose
-
-
-
?
4-nitrophenyl beta-D-fucoside + H2O
highest activity
723436
Avena sativa
4-nitrophenol + D-fucose
-
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
-
723436
Avena sativa
4-nitrophenol + D-galactose
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
-
723436
Avena sativa
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
lowest activity
723436
Avena sativa
4-nitrophenol + D-xylose
-
-
-
?
avenacoside A + H2O
-
723436
Avena sativa
26-desgluco-avenacoside A + D-glucose
-
-
-
?
avenacoside B + H2O
-
723436
Avena sativa
26-desgluco-avenacoside B + D-glucose
-
-
-
?
additional information
no activity with alpha-glycosides, beta-thioglucoside,cellobiose (beta1-4), gentiobiose (beta 1-6), laminaribiose (beta1-3), alpha-sophorose (beta1-2), 4-nitrophenyl-beta-D-xyloside, 3-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-galactoside, and 4-nitrophenyl-1-thio-beta-D-glucoside
723436
Avena sativa
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
pentamer or hexamer
5 or 6 * 60000, SDS-PAGE
Avena sativa
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
-
-
dissociation by freezing/thawing leads to complete loss of enzyme activity
Avena sativa
Other publictions for EC 3.2.1.188
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724918
Quidde
Evidence for three different s ...
Botrytis cinerea, Botrytis cinerea B05.10
Eur. J. Plant Pathol.
105
273-283
1999
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726124
Wubben
Detoxification of oat leaf sap ...
Parastagonospora avenae, Parastagonospora avenae WAC 1293
Phytopathology
86
986-992
1996
-
-
-
-
-
-
-
-
-
-
1
4
-
2
-
-
1
-
-
-
-
-
10
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
1
-
-
-
-
10
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
722249
Gus-Mayer
The amino acid sequence previo ...
Avena sativa
FEBS Lett.
347
51-54
1994
-
-
-
-
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
1
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723436
Gus-Mayer
Rüdiger, W.: Avenacosidase fr ...
Avena sativa
Plant Mol. Biol.
26
909-921
1994
-
-
-
-
-
-
-
-
1
-
2
2
-
4
-
-
1
-
-
4
1
-
9
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
2
-
-
-
1
-
4
1
-
9
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
726113
Grünweller
-
Characterization of a membrane ...
Avena sativa
Phytochemistry
24
1941-1943
1985
-
-
-
-
-
-
1
1
1
1
1
2
-
1
-
-
-
-
-
1
-
-
4
1
-
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
1
1
1
2
-
-
-
-
-
1
-
-
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-