Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli as a His-tagged fusion protein | Legionella pneumophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0365 | - |
UDP-N,N'-diacetylbacillosamine | pH 7.5, 37°C | Legionella pneumophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Legionella pneumophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Legionella pneumophila |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N,N'-diacetylbacillosamine + H2O = UDP + 2,4-diacetamido-2,4,6-trideoxy-D-mannopyranose | the reaction proceeds with an inversion of stereochemistry at C-2 and retention of stereochemistry at C-1. Evidence of the cleavage of the C-1-O bond and the C-2-H bond supports an elimination/hydration mechanism similar to that of NeuC in sialic acid bisoynthesis | Legionella pneumophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | when UDP-GlcNAc is incubated with the enzyme, no product formation is observed | Legionella pneumophila | ? | - |
? | |
UDP-N,N'-diacetylbacillosamine + H2O | - |
Legionella pneumophila | 2,4-diacetamido-2,4,6-trideoxymannose + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuC | - |
Legionella pneumophila |
UDP-N-acetylglucosamine 2-epimerase | - |
Legionella pneumophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Legionella pneumophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
59.1 | - |
UDP-N,N'-diacetylbacillosamine | pH 7.5, 37°C | Legionella pneumophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Legionella pneumophila |