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Literature summary for 3.2.1.183 extracted from

  • Chou, W.K.; Hinderlich, S.; Reutter, W.; Tanner, M.E.
    Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase (2003), J. Am. Chem. Soc., 125, 2455-2461.
    View publication on PubMed
Search for more literature for 3.2.1.183

Cloned(Commentary)

Cloned (Comment) Organism
UDP-GlcNAc 2-epimerase/ManNAc kinase from rat is overexpressed in Spodoptera frugiperda cells (Sf9 cells) using a baculovirus expression system Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a single ion exchange chromatographic step. The resulting protein is estimated to be 80% pure Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + H2O = N-acetyl-D-mannosamine + UDP the epimerase reaction is shown to proceed with a retention of stereochemistry at C-1, and the loss of UDP is found to occur via a C-O bond cleavage process. Results support a mechanism involving an anti-elimination of UDP followed by the syn-hydration of 2-acetamidoglucal Rattus norvegicus
a

Storage Stability

Storage Stability Organism
4°C, limited stability upon storage at 4 °C. The enzymatic activity is lost upon centrifugal concentration using ultrafiltration Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Rattus norvegicus N-acetyl-alpha-D-mannosamine + UDP
-
 ?

Synonyms

Synonyms Comment Organism
UDP-N-acetylglucosamine 2-epimerase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
 UDP-N-acetyl-alpha-D-glucosamine pH 7.5, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus