Literature summary for 3.2.1.176 extracted from

Taylor, L.E.; Knott, B.C.; Baker, J.O.; Alahuhta, P.M.; Hobdey, S.E.; Linger, J.G.; Lunin, V.V.; Amore, A.; Subramanian, V.; Podkaminer, K.; Xu, Q.; VanderWall, T.A.; Schuster, L.A.; Chaudhari, Y.B.; Adney, W.S.; Crowley, M.F.; Himmel, M.E.; Decker, S.R.; Beckham, G.T.
Engineering enhanced cellobiohydrolase activity (2018), Nat. Commun., 9, 1186 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using sodium phosphate monobasic monohydrate and 1.53 M potassium phosphate dibasic pH 4.0	Talaromyces funiculosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.195	-	4-nitrophenyl beta-D-lactopyranoside	at pH 4.0 and 55°C	Trichoderma reesei
2.656	-	4-nitrophenyl beta-D-lactopyranoside	at pH 4.0 and 60°C	Talaromyces funiculosus

Organism

Organism	UniProt	Comment	Textmining
Talaromyces funiculosus	F1CYZ0	-	-
Trichoderma reesei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Source 15Phe column chromatography	Trichoderma reesei
ammonium sulfate precipitation and Source 15Phe column chromatography	Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-lactopyranoside + H2O	-	Trichoderma reesei	4-nitrophenol + beta-D-lactopyranose	-	?
4-nitrophenyl beta-D-lactopyranoside + H2O	-	Talaromyces funiculosus	4-nitrophenol + beta-D-lactopyranose	-	?
pretreated corn stover + H2O	-	Trichoderma reesei	?	-	?
pretreated corn stover + H2O	-	Talaromyces funiculosus	?	-	?

Synonyms

Synonyms	Comment	Organism
CBH	-	Trichoderma reesei
CBH	-	Talaromyces funiculosus
Cel7A	-	Trichoderma reesei
Cel7A	-	Talaromyces funiculosus
cellobiohydrolase	-	Trichoderma reesei
cellobiohydrolase	-	Talaromyces funiculosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Trichoderma reesei
60	-	-	Talaromyces funiculosus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.238	-	4-nitrophenyl beta-D-lactopyranoside	at pH 4.0 and 55°C	Trichoderma reesei
0.273	-	4-nitrophenyl beta-D-lactopyranoside	at pH 4.0 and 60°C	Talaromyces funiculosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Trichoderma reesei
4	-	-	Talaromyces funiculosus

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Release 2023.1 (January 2023)