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Literature summary for 3.2.1.176 extracted from

  • Piens, K.; Stahlberg, J.; Nerinckx, W.; Teeri, T.T.; Claeyssens, M.
    Structure-reactivity studies of Trichoderma reesei cellobiohydrolase Cel7A (2004), Am. Chem. Soc. Symp. Ser., 889, 207-226.
No PubMed abstract available
Search for more literature for 3.2.1.176

Protein Variants

Protein Variants Comment Organism
D214N site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Trichoderma reesei
E212Q site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Trichoderma reesei
E217Q site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Trichoderma reesei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information detailed kinetic analysis of wild-type enzyme and mutants E212Q, D214N, and E217Q Trichoderma reesei
0.21
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.22
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
0.27
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.37
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
0.46
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
0.52
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
0.52
-
 4-bromophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
0.53
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.55
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
0.57
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
0.61
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.65
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.67
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
0.68
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.78
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.79
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Trichoderma reesei
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellulose + H2O Trichoderma reesei the enzyme hydrolyzes beta-1,4-linkages cellobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma reesei
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose double-displacement mechanism, transition states, formation of an glycosyl-enzyme intermediate, structure-reactivity studies, the catalytic triad is formed by Glu212, Asp214, and Glu217, the glycosylation step is rate-limiting, overview Trichoderma reesei
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl beta-cellobioside + H2O
-
 Trichoderma reesei 2,4-dinitrophenol + beta-cellobiose
-
 ?
2,4-dinitrophenyl beta-glucopyranoside + H2O
-
 Trichoderma reesei 2,4-dinitrophenol + beta-D-glucose
-
 ?
2,4-dinitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 2,4-dinitrophenol + beta-lactose
-
 ?
2-chloro-4-nitrophenyl beta-cellobioside + H2O
-
 Trichoderma reesei 2-chloro-4-nitrophenol + beta-cellobiose
-
 ?
2-chloro-4-nitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 2-chloro-4-nitrophenol + beta-lactose
-
 ?
4-bromophenyl beta-cellobioside + H2O
-
 Trichoderma reesei 4-bromophenol + beta-cellobiose
-
 ?
4-bromophenyl beta-glucopyranoside + H2O
-
 Trichoderma reesei 4-bromophenol + beta-D-glucose
-
 ?
4-bromophenyl beta-lactoside + H2O
-
 Trichoderma reesei 4-bromophenol + beta-lactose
-
 ?
4-methylumbelliferyl beta-lactoside + H2O
-
 Trichoderma reesei 4-methylumbelliferol + beta-lactose
-
 ?
4-nitrophenyl beta-cellobioside + H2O
-
 Trichoderma reesei 4-nitrophenol + beta-cellobiose
-
 ?
4-nitrophenyl beta-glucopyranoside + H2O
-
 Trichoderma reesei 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-lactoside + H2O
-
 Trichoderma reesei 4-nitrophenol + beta-lactose
-
 ?
cellulose + H2O the enzyme hydrolyzes beta-1,4-linkages Trichoderma reesei cellobiose + ?
-
 ?
additional information structure-reactivity studies Trichoderma reesei ?
-
 ?

Subunits

Subunits Comment Organism
More structure-reactivity studies of wild-type enzyme and mutants E212Q, D214N, and E217Q, preparation of enzyme catalytic core domains of wild-type and mutant enzymes by papain treatment Trichoderma reesei

Synonyms

Synonyms Comment Organism
Cel7A
-
 Trichoderma reesei
cellobiohydrolase
-
 Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00066
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.0041
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.0063
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.0073
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.035
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
0.042
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
0.063
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant E212Q Trichoderma reesei
0.15
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
1.7
-
 4-bromophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
2.3
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
2.9
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, mutant D214N Trichoderma reesei
3.5
-
 4-nitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
12.2
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, mutant E217Q Trichoderma reesei
12.8
-
 2-chloro-4-nitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
47
-
 4-methylumbelliferyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei
104
-
 2,4-dinitrophenyl beta-lactoside pH 5.7, 37°C, wild-type enzyme Trichoderma reesei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7
-
 assay at Trichoderma reesei