Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Sodium acetate | 0.1 M slowly inactivates the enzyme: at 0.5 M 33% residual activity, at 1.1 M 10% residual activity | Aspergillus aculeatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, SDS-PAGE | Aspergillus aculeatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus aculeatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus aculeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rhamnogalacturonan I oligosaccharides with D-galacturonic acid at the reducing end and L-rhamnopyranose at the nonreducing end + H2O | alpha-L-Rha-(1->4)-[alpha-D-GalA-(1->2)-alpha-L-Rha]n-(1->4)-alpha-D-GalA. The enzyme is hindered when the terminal Rha residue is substituted at the 4-position by a beta-D-galactose. No particular preference of the enzyme for low or high molecular mass rhamnogalacturonan fragments | Aspergillus aculeatus | beta-L-rhamnopyranose + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing end | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE | Aspergillus aculeatus |
Synonyms | Comment | Organism |
---|---|---|
RG alpha-L-rhamnopyranohydrolase | - |
Aspergillus aculeatus |
RG-rhamnohydrolase | - |
Aspergillus aculeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
sodium acetate buffer | Aspergillus aculeatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h up to temperatures of 60°C in 50 mM sodium acetate buffer (pH 5) | Aspergillus aculeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
McIlvaine buffer | Aspergillus aculeatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | stable | Aspergillus aculeatus |