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Literature summary for 3.2.1.168 extracted from

  • Minig, M.; Mazzaferro, L.S.; Erra-Balsells, R.; Petroselli, G.; Breccia, J.D.
    alpha-Rhamnosyl-beta-glucosidase-catalyzed reactions for analysis and biotransformations of plant-based foods (2011), J. Agric. Food Chem., 59, 11238-11243.
    View publication on PubMed
Search for more literature for 3.2.1.168

Application

Application Comment Organism
food industry potential use of the enzyme for biotechnoligical applications, e.g. in aroma modulation of fermented foods Acremonium sp.

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol hydrolysis only in the presence of 12% ethanol, no enzyme activity in aqueous medium Acremonium sp.

Organism

Organism UniProt Comment Textmining
Acremonium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, fractionation with ammonium sulfate and two chromatographic steps: butyl-agarose and QAE-Sephadex Acremonium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenethyl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 3.8% in 12% ethanol Acremonium sp. 2-phenylethanol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
geranyl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 2.9% in 12% ethanol Acremonium sp. geraniol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?
hesperidin + H2O purified aromatic and terpenyl rutinosides are incubated as substrates, 14.3% relative activity in aqueous medium, 21.7% in 12% ethanol Acremonium sp. hesperetin + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside i.e. hesperetin + rutinose ?
additional information hydrolysis not detected in aqueous medium without ethanol Acremonium sp. ?
-
 ?
neryl rutinoside + H2O purified aromatic an terpenyl rutinosides are incubated as substrates, no activity in aqueous medium, 2.9% in 12% ethanol Acremonium sp. nerol + 6-O-alpha-L-rhamnopyranosyl-beta-D-glucopyranoside
-
 ?

Synonyms

Synonyms Comment Organism
alpha-rhamnosyl-beta-glucosidase
-
 Acremonium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Acremonium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Acremonium sp.