BRENDA - Enzyme Database show
show all sequences of 3.2.1.162

Degradation of lambda-carrageenan by Pseudoalteromonas carrageenovora lambda-carrageenase: a new family of glycoside hydrolases unrelated to kappa- and iota-carrageenases

Guibet, M.; Colin, S.; Barbeyron, T.; Genicot, S.; Kloareg, B.; Michel, G.; Helbert, W.; Biochem. J. 404, 105-114 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea; expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea
Pseudoalteromonas carrageenovora
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Pseudoalteromonas carrageenovora
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78000
-
gel filtration
Pseudoalteromonas carrageenovora
97000
-
1 * 97000, SDS-PAGE; SDS-PAGE
Pseudoalteromonas carrageenovora
105000
-
pre-protein, calculated from the deduced amino acid sequence
Pseudoalteromonas carrageenovora
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lambda-carrageenan + H2O
Pseudoalteromonas carrageenovora
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
?
lambda-carrageenan + H2O
Pseudoalteromonas carrageenovora ATCC 43555
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudoalteromonas carrageenovora
-
; ATCC43555
-
Pseudoalteromonas carrageenovora
Q0JRK4
-
-
Pseudoalteromonas carrageenovora ATCC 43555
-
ATCC43555
-
Purification (Commentary)
Commentary
Organism
; from host strain
Pseudoalteromonas carrageenovora
Reaction
Reaction
Commentary
Organism
Endohydrolysis of (1->4)-beta-linkages in the backbone of lambda-carrageenan, resulting in the tetrasaccharide alpha-D-Gal-p2,6S2-(1->3)-beta-D-Gal-p2S-(1->4)-alpha-D-Gal-p2,6S2-(1->3)-D-Gal-p2-S
proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration
Pseudoalteromonas carrageenovora
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture medium
-
Pseudoalteromonas carrageenovora
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lambda-carrageenan + H2O
-
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer
-
-
?
lambda-carrageenan + H2O
CglA hydrolase proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
neo-lambda-carratetraose is the main degradation product
-
-
?
lambda-carrageenan + H2O
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
-
?
lambda-carrageenan + H2O
-
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer
-
-
?
lambda-carrageenan + H2O
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
-
?
neo-lambda-carrahexaose + H2O
-
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrabiose
-
-
-
?
neo-lambda-carrahexaose + H2O
-
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrabiose
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 97000, SDS-PAGE
Pseudoalteromonas carrageenovora
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea
Pseudoalteromonas carrageenovora
expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea
Pseudoalteromonas carrageenovora
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
extracellular
-
Pseudoalteromonas carrageenovora
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
78000
-
gel filtration
Pseudoalteromonas carrageenovora
97000
-
SDS-PAGE
Pseudoalteromonas carrageenovora
97000
-
1 * 97000, SDS-PAGE
Pseudoalteromonas carrageenovora
105000
-
pre-protein, calculated from the deduced amino acid sequence
Pseudoalteromonas carrageenovora
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
lambda-carrageenan + H2O
Pseudoalteromonas carrageenovora
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
?
lambda-carrageenan + H2O
Pseudoalteromonas carrageenovora ATCC 43555
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudoalteromonas carrageenovora
from host strain
Pseudoalteromonas carrageenovora
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture medium
-
Pseudoalteromonas carrageenovora
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
lambda-carrageenan + H2O
-
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer
-
-
?
lambda-carrageenan + H2O
CglA hydrolase proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
neo-lambda-carratetraose is the main degradation product
-
-
?
lambda-carrageenan + H2O
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
-
?
lambda-carrageenan + H2O
-
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer
-
-
?
lambda-carrageenan + H2O
sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrahexaose
main reaction products
-
-
?
neo-lambda-carrahexaose + H2O
-
667539
Pseudoalteromonas carrageenovora
neo-lambda-carratetraose + neo-lambda-carrabiose
-
-
-
?
neo-lambda-carrahexaose + H2O
-
667539
Pseudoalteromonas carrageenovora ATCC 43555
neo-lambda-carratetraose + neo-lambda-carrabiose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 97000, SDS-PAGE
Pseudoalteromonas carrageenovora
Other publictions for EC 3.2.1.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731525
Li
Purification and characterizat ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Lc50-1
Biotechnol. Lett.
36
1669-1674
2014
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-
-
-
-
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7
-
1
4
1
2
-
4
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-
1
-
-
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1
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
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7
-
-
1
4
1
2
-
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1
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1
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2
1
1
-
1
-
1
-
1
-
-
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-
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-
-
732212
Kang
Efficient enzymatic degradatio ...
Pseudoalteromonas carrageenovora
J. Biotechnol.
192 Pt A
108-113
2014
-
1
1
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1
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-
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1
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1
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-
1
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-
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-
1
1
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-
1
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-
-
-
-
-
1
1
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1
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1
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1
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-
-
-
-
1
1
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1
-
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-
-
-
-
-
-
-
667539
Guibet
Degradation of lambda-carragee ...
Pseudoalteromonas carrageenovora, Pseudoalteromonas carrageenovora ATCC 43555
Biochem. J.
404
105-114
2007
-
-
1
-
-
-
-
-
1
-
3
2
-
4
-
-
1
1
-
1
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-
7
1
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2
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1
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4
2
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2
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1
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7
1
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-
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668168
Guibet
Complete assignment of 1H and ...
Pseudoalteromonas carrageenovora
Carbohydr. Res.
341
1859-1869
2006
-
-
-
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-
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3
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1
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1
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669161
Ohta
A novel enzyme, lambda-carrage ...
Pseudoalteromonas sp., Pseudoalteromonas sp. CL19
J. Biochem.
140
475-481
2006
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1
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1
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5
1
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3
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1
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1
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2
1
1
1
2
-
1
1
-
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1
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1
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5
1
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1
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1
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2
1
1
1
2
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1
1
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668129
Johnston
Enzymic hydrolysis of the pota ...
Pseudomonas carrageenovora
Can. J. Microbiol.
19
779-788
1973
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1
2
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1
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1
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1
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