BRENDA - Reference to 3.2.1.162; Id = 667539

expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea; expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea

Pseudoalteromonas carrageenovora

extracellular

-

Pseudoalteromonas carrageenovora

-

78000

-

gel filtration

Pseudoalteromonas carrageenovora

97000

-

1 * 97000, SDS-PAGE; SDS-PAGE

Pseudoalteromonas carrageenovora

105000

-

pre-protein, calculated from the deduced amino acid sequence

Pseudoalteromonas carrageenovora

lambda-carrageenan + H2O

Pseudoalteromonas carrageenovora

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

lambda-carrageenan + H2O

Pseudoalteromonas carrageenovora ATCC 43555

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

Pseudoalteromonas carrageenovora

-

; ATCC43555

-

Pseudoalteromonas carrageenovora

Q0JRK4

-

Pseudoalteromonas carrageenovora ATCC 43555

-

ATCC43555

-

; from host strain

Pseudoalteromonas carrageenovora

Endohydrolysis of (1->4)-beta-linkages in the backbone of lambda-carrageenan, resulting in the tetrasaccharide alpha-D-Gal-p2,6S2-(1->3)-beta-D-Gal-p2S-(1->4)-alpha-D-Gal-p2,6S2-(1->3)-D-Gal-p2-S

proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration

Pseudoalteromonas carrageenovora

culture medium

-

Pseudoalteromonas carrageenovora

-

lambda-carrageenan + H2O

-

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer

-

?

lambda-carrageenan + H2O

CglA hydrolase proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

neo-lambda-carratetraose is the main degradation product

-

?

lambda-carrageenan + H2O

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

lambda-carrageenan + H2O

-

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer

-

?

lambda-carrageenan + H2O

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

neo-lambda-carrahexaose + H2O

-

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrabiose

-

?

neo-lambda-carrahexaose + H2O

-

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrabiose

-

?

monomer

1 * 97000, SDS-PAGE

Pseudoalteromonas carrageenovora

CglA

-

Pseudoalteromonas carrageenovora

CglA hydrolase

-

Pseudoalteromonas carrageenovora

expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea

Pseudoalteromonas carrageenovora

expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea

Pseudoalteromonas carrageenovora

extracellular

-

Pseudoalteromonas carrageenovora

-

78000

-

gel filtration

Pseudoalteromonas carrageenovora

97000

-

SDS-PAGE

Pseudoalteromonas carrageenovora

97000

-

1 * 97000, SDS-PAGE

Pseudoalteromonas carrageenovora

105000

-

pre-protein, calculated from the deduced amino acid sequence

Pseudoalteromonas carrageenovora

lambda-carrageenan + H2O

Pseudoalteromonas carrageenovora

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

lambda-carrageenan + H2O

Pseudoalteromonas carrageenovora ATCC 43555

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

-

Pseudoalteromonas carrageenovora

from host strain

Pseudoalteromonas carrageenovora

culture medium

-

Pseudoalteromonas carrageenovora

-

lambda-carrageenan + H2O

-

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer

-

?

lambda-carrageenan + H2O

CglA hydrolase proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

neo-lambda-carratetraose is the main degradation product

-

?

lambda-carrageenan + H2O

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

lambda-carrageenan + H2O

-

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer

-

?

lambda-carrageenan + H2O

sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrahexaose

main reaction products

-

?

neo-lambda-carrahexaose + H2O

-

667539

Pseudoalteromonas carrageenovora

neo-lambda-carratetraose + neo-lambda-carrabiose

-

?

neo-lambda-carrahexaose + H2O

-

667539

Pseudoalteromonas carrageenovora ATCC 43555

neo-lambda-carratetraose + neo-lambda-carrabiose

-

?

monomer

1 * 97000, SDS-PAGE

Pseudoalteromonas carrageenovora

753336

Zhu

Insight into carrageenases ma ...

Pseudoalteromonas carrageenovora, Pseudoalteromonas sp. CL19

Crit. Rev. Biotechnol.

38

1261-1276

2018

-

2

-

2

-

2

-

4

-

5

2

-

2

-

2

-

2

-

4

-

2

-

2

-

2

-

755018

Ghanbarzadeh

-

Carrageenans and carrageenase ...

Bacillus sp. Lc50-1

Phytochem. Rev.

17

535-571

2018

-

2

-

1

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

1

-

1

-

2

-

2

-

1

-

1

-

2

-

731525

Li

Purification and characterizat ...

Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) Lc50-1

Biotechnol. Lett.

36

1669-1674

2014

-

7

-

1

4

1

2

-

5

-

1

-

1

-

2

1

3

1

-

1

-

1

-

1

-

7

-

1

4

1

2

-

1

-

1

-

2

1

-

1

-

1

-

1

-

732212

Kang

Efficient enzymatic degradatio ...

Pseudoalteromonas carrageenovora

J. Biotechnol.

192 Pt A

108-113

2014

-

1

-

1

-

1

-

1

-

1

-

1

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

667539

Guibet

Degradation of lambda-carragee ...

Pseudoalteromonas carrageenovora, Pseudoalteromonas carrageenovora ATCC 43555

Biochem. J.

404

105-114

2007

-

1

-

1

-

3

2

-

4

-

1

-

1

-

7

1

2

-

2

-

1

-

4

2

-

2

-

1

-

7

1

-

668168

Guibet

Complete assignment of 1H and ...

Pseudoalteromonas carrageenovora

Carbohydr. Res.

341

1859-1869

2006

-

3

-

1

-

1

-

669161

Ohta

A novel enzyme, lambda-carrage ...

Pseudoalteromonas sp.

J. Biochem.

140

475-481

2006

-

1

-

1

-

5

1

-

3

-

1

-

1

-

1

2

-

1

-

1

-

1

-

5

1

-

1

-

1

-

1

2

-

1

-

668129

Johnston

Enzymic hydrolysis of the pota ...

Pseudomonas carrageenovora

Can. J. Microbiol.

19

779-788

1973

-

1

2

-

1

-

1

-

1

-

1

-

2

-

1

-

1

-

Degradation of lambda-carrageenan by Pseudoalteromonas carrageenovora lambda-carrageenase: a new family of glycoside hydrolases unrelated to kappa- and iota-carrageenases

Data extracted from this reference: