Cloned (Comment) | Organism |
---|---|
cDNA library screening, gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Lolium perenne |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | the enzyme is 70-90% inhibited by 10-40 mM sucrose | Lolium perenne |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,6-kestotetraose + H2O | Lolium perenne | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium perenne | D0PQE8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains five potential N-glycosylation sites | Lolium perenne |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | in elongating leaf bases and mature leaf sheaths | Lolium perenne | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1-kestotetraose + H2O | low activity | Lolium perenne | ? | - |
? | |
1-kestotriose + H2O | low activity | Lolium perenne | ? | - |
? | |
6,6-kestotetraose + H2O | - |
Lolium perenne | ? | - |
? | |
6-kestotriose + H2O | best substrate | Lolium perenne | ? | - |
? | |
6G-kestotriose + H2O | - |
Lolium perenne | ? | - |
? | |
fructan + H2O | - |
Lolium perenne | ? | - |
? | |
levan + H2O | from Erwinia herbicola | Lolium perenne | ? | - |
? | |
additional information | purification of and assay with fructans from Lolium perenne, overview | Lolium perenne | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-FEH | - |
Lolium perenne |
6-FEHa | - |
Lolium perenne |
6-fructan exohydrolase | - |
Lolium perenne |
Lp6-FEHa | - |
Lolium perenne |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme's the catalytic triad is conserved among glycoside hydrolase family 32, GH32, members | Lolium perenne |
metabolism | 6-FEHs are key enzymes associated with perennity of forage species | Lolium perenne |
physiological function | Lolium perenne is a major forage grass species that accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases (FEHs), sustaining regrowth following defoliation. Differences in the regulation of FEH activity among forage grasses influence their tolerance to defoliation | Lolium perenne |