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Literature summary for 3.2.1.153 extracted from
- Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold (2012), J. Plant Physiol., 169, 294-302.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Wfh-sm3, recombinant expression in Pichia pastoris | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Triticum aestivum | isozyme Wfh-sm3m hydrolyzes beta-(2,6)-linkages of fructans (6-kestotriose) more efficiently than beta-(2,1)-linkages of fructans (1-kestotriose and 1,1-kestotetraose). On the other hand, Wfh-sm3m cannot hydrolyze long-chain fructans such as inulin and bacterial levan | ? | - |
? |  |
| wheat graminan + H2O | Triticum aestivum | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
cultivars PI 173438 (snow mold-resistant) and Valuevskaya (snow mold-susceptible), gene Wfh-sm3 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,1-kestotetraose + H2O | - |
Triticum aestivum | ? | - |
? | |
| 1-kestotriose + H2O | - |
Triticum aestivum | ? | - |
? | |
| 6-kestotriose + H2O | - |
Triticum aestivum | ? | - |
? | |
| additional information | isozyme Wfh-sm3m hydrolyzes beta-(2,6)-linkages of fructans (6-kestotriose) more efficiently than beta-(2,1)-linkages of fructans (1-kestotriose and 1,1-kestotetraose). On the other hand, Wfh-sm3m cannot hydrolyze long-chain fructans such as inulin and bacterial levan | Triticum aestivum | ? | - |
? | |
| additional information | wheat graminan degradation by Wfh-sm3 recombinant protein Wfh-sm3m. The recombinant protein Wfh-sm3m preferentially degrades 6-kestotriose and possesses minor hydrolase activity to 1-kestotriose and 1,1-kestotetraose, poor activity with sucrose, and no activity with inulin and levan | Triticum aestivum | ? | - |
? | |
| wheat graminan + H2O | - |
Triticum aestivum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-FEH | - |
Triticum aestivum |
| FEH | - |
Triticum aestivum |
| fructan exohydrolase | - |
Triticum aestivum |
| Wfh-sm3m | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 35 | assay at | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Triticum aestivum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | snow molds consume carbon sources contained in the inoculated wheat tissues but cannot effectively use wheat polysaccharide including fructans compared with mono- and disaccharides. Therefore, in snow mold inoculated wheat tissues, fructans are rapidly degraded, mainly by wheat enzymes, FEHs, to maintain necessary levels of mono- and disaccharides for metabolic demands under snow cover | Triticum aestivum |
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