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Literature summary for 3.2.1.153 extracted from
- Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus (2009), Planta, 231, 13-25.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Bromus pictus |
| single copy gene Bp1-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Pichia pastoris | Bromus pictus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | about 50% decreased enzyme activity in the first leaf section after 7 days of cold treatment, fructan accumulation profile along with cold treatment, overview | Bromus pictus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bromus pictus | Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bromus pictus | - |
a cold tolerant, graminean decaploid species from the Patagonian region of Argentina, gene Bp1-FEHa | - |
| Bromus pictus | D2IGW7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Bromus pictus | - |
| leaf | meature leaves, highest Bp1-FEHa expression levels in the second section of the sheath and the tip of the blade | Bromus pictus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestotriose + H2O | 1-FEHa is a fructan exohydrolase with predominant activity towards beta-(2-1) linkages | Bromus pictus | ? | - |
? | |
| 6-kestotriose + H2O | lower activity compared to 1-kestotriose | Bromus pictus | ? | - |
? | |
| additional information | Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages | Bromus pictus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-FEH | - |
Bromus pictus |
| 1-FEHa | - |
Bromus pictus |
| fructan 1-exohydrolase | - |
Bromus pictus |
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