Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium | Saccharomyces paradoxus |
overexpression in Saccharomyces cerevisiae strain VIN13, which is itself completely devoid of endo-polygalacturonase activity, and secretion to the medium | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P47180 | gene PGU1 | - |
Saccharomyces cerevisiae BY4742 | P47180 | gene PGU1 | - |
Saccharomyces paradoxus | F5B9B9 | gene PGU1 | - |
Saccharomyces paradoxus RO88 | F5B9B9 | gene PGU1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | - |
Saccharomyces cerevisiae | galacturonic acid + ? | - |
? | |
polygalacturonic acid + H2O | - |
Saccharomyces paradoxus | galacturonic acid + ? | - |
? | |
polygalacturonic acid + H2O | - |
Saccharomyces cerevisiae BY4742 | galacturonic acid + ? | - |
? | |
polygalacturonic acid + H2O | - |
Saccharomyces paradoxus RO88 | galacturonic acid + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-polygalacturonase | - |
Saccharomyces cerevisiae |
endo-polygalacturonase | - |
Saccharomyces paradoxus |
pectinase | - |
Saccharomyces cerevisiae |
pectinase | - |
Saccharomyces paradoxus |
Pgu1 | - |
Saccharomyces cerevisiae |
Pgu1 | - |
Saccharomyces paradoxus |
ScPgu1 | - |
Saccharomyces cerevisiae |
SpPgu1 | - |
Saccharomyces paradoxus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Saccharomyces cerevisiae |
45 | - |
- |
Saccharomyces paradoxus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25% of maximal activity at 25°C, maximal activity at 45°C | Saccharomyces paradoxus |
25 | 45 | 30% of maximal activity at 25°C, maximal activity at 45°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Saccharomyces cerevisiae |
5.5 | - |
- |
Saccharomyces paradoxus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4.5 | 79% of maximal activity at pH 3.5, maximal activity at pH 4.5 | Saccharomyces cerevisiae |
3.5 | 5.5 | 43% of maximal activity at pH 3.5, maximal activity at pH 5.5 | Saccharomyces paradoxus |
General Information | Comment | Organism |
---|---|---|
additional information | comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions | Saccharomyces cerevisiae |
additional information | comparison of endo-polygalacturonase from Saccharomyces cerevisiae with the enzyme from Saccharomyces paradoxus after overexpression in Saccharomyces cerevisiae under wine production conditions, overview. The higher activity of Saccharomyces paradoxus strains under laboratory conditions might be due to a different regulation mechanism rather than to a different sequence of gene PGU1. ScPgu1 displayed higher Km and Vmax than SpPgu1 thereby showing a slightly lower affinity for the substrate but a twofold stronger activity under optimal conditions | Saccharomyces paradoxus |