KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for polygalacturonate is 1.03 g/l with polygalacturonase I. Km value for polygalacturonate is 1.03 g/L with polygalacturonase II | Aspergillus japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | maximal activity at 0.1-0.2 M, polygalacturonase I and II | Aspergillus japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, polygalacturonase I, SDS-PAGE | Aspergillus japonicus |
65000 | - |
x * 65000, polygalacturonase II, SDS-PAGE | Aspergillus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate + H2O | - |
Aspergillus japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, polygalacturonase I, SDS-PAGE | Aspergillus japonicus |
? | x * 65000, polygalacturonase II, SDS-PAGE | Aspergillus japonicus |
Synonyms | Comment | Organism |
---|---|---|
endo-polygalacturonase I | - |
Aspergillus japonicus |
endo-polygalacturonase II | - |
Aspergillus japonicus |
polygalacturonase I | - |
Aspergillus japonicus |
polygalacturonase II | - |
Aspergillus japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
about 40% inactivation of polygalacturonase I after 60 min, about 10% inactivation of polygalacturonase II after 60 min | Aspergillus japonicus |
40 | - |
about 55% inactivation of polygalacturonase I after 60 min, about 50% inactivation of polygalacturonase II after 60 min | Aspergillus japonicus |
50 | - |
complete inactivation of polygalacturonase I after 10 min, complete inactivation of polygalacturonase II after 40 min | Aspergillus japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
Polygalacturonate | polygalacturonase II | Aspergillus japonicus | |
90 | - |
Polygalacturonate | polygalacturonase I | Aspergillus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
polygalacturonase II | Aspergillus japonicus |
5 | - |
polygalacturonase I | Aspergillus japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | pH 3.5: about 50% of maximal activity, pH 5.5: about 60% of maximal activity, polygalacturonase II | Aspergillus japonicus |
4.5 | 5.5 | pH 4.5: about 50% of maximal activity, pH 5.5: about 40% of maximal activity, polygalacturonase I | Aspergillus japonicus |