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Literature summary for 3.2.1.147 extracted from

  • Wade, K.; Ito, Y.; Ramarathnam, A.; Holtzclaw, W.; Faheya, J.
    Purification of active myrosinase from plants by aqueous two-phase counter-current chromatography (2015), Phytochem. Anal., 26, 47-53 .
No PubMed abstract available
Search for more literature for 3.2.1.147

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. italica A0A343IQS8
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Moringa oleifera
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 leaves are collected in California (Moringa Farms, Sherman Oaks, CA, USA)
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Raphanus sativus V9PVN6
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Sinapis alba
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Purification (Commentary)

Purification (Comment) Organism
rapid isolation of catalytically active plant myrosinase from 3-day-old sprouts at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation Brassica oleracea var. italica
rapid isolation of catalytically active plant myrosinase from 7-day-old sprouts at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation Raphanus sativus
rapid isolation of catalytically active plant myrosinase from fresh leaves at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation Moringa oleifera
rapid isolation of catalytically active plant myrosinase from fresh seed powder at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
leaf
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 Moringa oleifera
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seed
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 Sinapis alba
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sprout
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 Brassica oleracea var. italica
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sprout
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 Raphanus sativus
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Subunits

Subunits Comment Organism
? x * 63000, about, SDS-PAGE Sinapis alba
? x * 63000, about, SDS-PAGE Moringa oleifera
? x * 63000, about, SDS-PAGE Brassica oleracea var. italica
? x * 63000, about, SDS-PAGE Raphanus sativus

Synonyms

Synonyms Comment Organism
myrosinase
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 Sinapis alba
myrosinase
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 Moringa oleifera
myrosinase
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 Brassica oleracea var. italica
myrosinase
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 Raphanus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
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 assay at room temperature Sinapis alba
22
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 assay at room temperature Moringa oleifera
22
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 assay at room temperature Brassica oleracea var. italica
22
-
 assay at room temperature Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
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 assay at Sinapis alba
6
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 assay at Moringa oleifera
6
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 assay at Brassica oleracea var. italica
6
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 assay at Raphanus sativus

General Information

General Information Comment Organism
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulphate) precursors Sinapis alba
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulphate) precursors Moringa oleifera
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulphate) precursors Brassica oleracea var. italica
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulphate) precursors. Myrosinase binding proteins and/or myrosinase-associated proteins are critical parts of the myrosinase complex, and are required for full enzyme activity Raphanus sativus